旋磁场对锂-匹鲁卡品癫痫大鼠模型的影响

目的 观察旋磁场对锂-匹鲁卡品癫痫大鼠模型癫痫发作和海马mGluR1、mGluR5表达的影响。方法 将癫痫组大鼠按60mg／kg体重腹腔注射氯化锂24h后，再给予匹鲁卡品（35mg／kg体重）腹腔注射致痫。观察旋磁场（场强为20mT）作用不同时间后对癫痫大鼠模型癫痫发作程度、潜伏期及终止时间的影响．并同时记录癫痫发作时的伴随症状及脑电数据；分析旋磁场对海马mGluR1、mGluR5表达的影响。用电泳图像分析系统计算mGluR1、mGluR5mRNA水平的相对含量，计算mGluR1mRNA与mGluR5mRNA的比值。结果 经为期8d的旋磁场作用后，癫痫大鼠发作终止时间明显提前，发作次数减少，其伴随症状减轻；模型组大鼠mGluR1mRNA水平明显增高，mGluR5mRNA水平明显降低，其mGluR1与mGluR5比值明显大于其它各组；各磁场作用组癫痫大鼠模型的基因表达与模型组正好相反，其mGluR5水平明显增高而mGluR1水平显著降低，尤其是经磁场作用8d后，这种变化更加显著。结论 20mT旋磁场对大鼠癫痫发作有一定的抑制作用，长时间的磁场作用对癫痫大鼠海马mGluR1mRNA和mGluR5mRNA表达有显著影响，但mGluR5表达增强的意义还有待进一步探讨。

The effects of a rotating magnetic field on Li pilocarpine-induced seizures in rats

Objective To study the effects of a rotating magnetic field(RMF)on Li pilocarpine-in- duced seizure activity and the expression of mGluR1 and mGluR5 in the hippocampus.Methods Thirty rats were divided into 5 groups.Each rat in the model(M),short treatment(ST)and long treatment(LT)groups was treated with intra-peritoneal injections of lithium chloride(60 mg/kg),followed by an intra-peritoneal injec- tion of pilocarpine(35 mg/kg)24 h later.The rats in the ST group were exposed to 20 mT RMF for 20 min ev- ery day for 3 d before seizure induction,while the rats in LT group were exposed to the same RMF for 8 d.The latency,severity and duration of seizure,as well as accompanying symptoms and electroencephalogram data, were recorded,and the expression of mGluR1 and mGluR5 was calculated using an electrophoretic imaging anal- ysis system.Results The duration,times and accompanying symptoms of seizure were significantly decreased in the LT group.The mGluR1 mRNA level and mGluR1/mGluR5 ratio in the M group were markedly increased, but the mGluR5 mRNA level was obviously decreased,while the expression of mGluR1 in the ST and LT groups was decreased,and mGluR5 was increased.Conclusions Seizure activity in rats can be inhibited by 20 mT RMF,and the expression of mGluRl and mGluR5 in the hippocampus of rats suffering seizures can be markedly influenced by longer-term RMF.