石蜡油几种灭菌方法的比较试验

目的 探讨最佳的石蜡油灭菌方法。方法采用载体定性杀菌试验方法，对石蜡油分别用下排气式压力蒸汽灭菌、干热灭菌、Co^60γ射线辐照灭菌的多种方法进行测试并比较其结果。结果压力蒸汽灭菌法不管是延长灭菌时间、还是采用间歇灭菌法均无法达到100％的灭菌效果；干热灭菌和Co^60γ射线辐照灭菌，均无菌生长。结论石蜡油灭菌方式以干热灭菌和辐照灭菌效果可靠，但其包装、操作、设备等方面都需要严格要求，压力蒸汽灭菌法不可取。

Several Sterilizing Methods for Paraffin Oil:A Comparative Experiment

OBJECTIVE To study effective and convenient method for paraffin oil sterilization. METHODS By using carrier qualitative germicidal test, to compare pressure steam sterilization, dry heat sterilization and cobalt-60 gamma-ray radiation sterilization to test the sterilizing effect and operating procedure. RESULTS Pressure steam sterilization was unable to achieve 100% sterilizing effect, whether we extended the time or use the intermittent sterilization. After dry heat or radiation sterilization processes, no microorganism was found. CONCLUSIONS Effect of sterilization with dry heat or radiation sterilization is trustable, but its packing, operation and equipment are requested strictly, and pressure steam sterilization may be not good for paraffin oil.