| 新疆博乐地区动物感染恙虫病东方体调查研究 |
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| 引用本文： | 党荣理,任立松,高金拽,马德新,董路宁.新疆博乐地区动物感染恙虫病东方体调查研究[J].中国预防医学杂志,2011(5):391-393. |
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| 作者姓名： | 党荣理 任立松 高金拽 马德新 董路宁 |
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| 作者单位： | 新疆军区疾病预防控制中心; |
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| 基金项目： | 总后卫生部“十一五”第二批基金项目(08G014) |
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| 摘 要： | 目的了解新疆博乐地区动物自然感染恙虫病东方体(Ot)的情况。方法捕鼠、旱獭取脾组织,采用颈部静脉刺穿法取羊血,提取DNA,应用巢式PCR(nPCR)检测Ot-Sta56基因;对部分阳性样本测序,通过NCBI网站对序列进行BLAST比较分析。结果在博乐地区平原湿地、农田和居民区捕鼠126只,其中7只nPCR检测阳性,阳性率5.56%;取绵羊群血210份,8份nPCR检测阳性,阳性率为3.81%;捕获鸟类12只,其中2只nPCR检测阳性,阳性率为16.67%。在山地草原捕获鼠58只,捕获旱獭26只,nPCR检测无阳性。DNA序列分析表明,鼠类、鸟类、羊血阳性样本nPCR产物碱基序列相同,与Karp株相应DNA片段的碱基序列同源性最高,均为97%,应属于Karp型。结论在新疆博乐地区平原湿地中存在恙虫病东方体感染,为下一步深入进行该地区恙虫病东方体疫源地的调查奠定了基础。
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| 关 键 词： | 恙虫病东方体 恙虫病 流行病学 |
Investigation on animal natural infection of Orientia tsutsugamushi in some areas of Bole area in Xinjiang,China |
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| DANG Rong-li,REN Li-song,GAO Jin-zhuai,MA De-xin,DONG Lu-ning.Xinjiang Comm, Center for Disease Control , Prevention,Urumqi ,China.Investigation on animal natural infection of Orientia tsutsugamushi in some areas of Bole area in Xinjiang,China[J].China Preventive Medicine,2011(5):391-393. |
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| Authors: | DANG Rong-li REN Li-song GAO Jin-zhuai MA De-xin DONG Lu-ningXinjiang Comm Center for Disease Control Prevention Urumqi China |
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| Institution: | DANG Rong-li,REN Li-song,GAO Jin-zhuai,MA De-xin,DONG Lu-ning.Xinjiang Command Center for Disease Control and Prevention,Urumqi 830011,China |
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| Abstract: | Objective To investigate animal natural infection of Orientia tsutsugamushi(Ot)in Bole area of Xinjiang,China.Methods Internal jugular vein puncture was used to collect blood and extract DNA from spleens of the captured mice,nested-polymerase chain reaction(nPCR) technique was used to detect the Ot-Sta 56 gene.Some positive samples were sequenced and analyzed in BLAST through NCBI website.Results A total of 154 rodents,12 birds and 26 woodchucks were captured in Bole district,Xinjiang uyghur Autonomous Regi... |
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| Keywords: | Orientia tsutsugamushi Scrub typhus Epidemiology |
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