Antimicrobial action of four herbal plants over mixed-species biofilms of Candida albicans with four different microorganisms |
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| Authors: | Nádia Domingues DDS MSc PhD Lucas de Paula Ramos MSc PhD Larissa Marques Pereira DDS Pâmela Beatriz do Rosário Estevam dos Santos MSc Liliana Scorzoni MSc PhD Thaís Cristine Pereira MSc Amjad Abu Hasna DDS MSc PhD Cláudio Antonio Talge Carvalho DDS MSc PhD Luciane Dias de Oliveira DDS MSc PhD |
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| Affiliation: | 1. Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University – UNESP, São José dos Campos, Brazil;2. Department of Restorative Dentistry, Endodontics Division, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, SP, Brazil |
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| Abstract: | This study aimed to evaluate the antimicrobial effect of four herbal plants glycolic extracts over mixed-species biofilm composed of Candida albicans (C. albicans) and another pathogenic bacterium as alternative therapy to be investigated. Four plants extract of Pfaffia paniculata roots; Hamamelis virginiana leaf, Stryphnodendron barbatiman tree bark and Gymnema sylvestre stem and leaves were tested over multi-species biofilm of C. albicans (ATCC 18804) and Streptococcus mutans (ATCC 35688), Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 4083) or Pseudomonas aeruginosa (ATCC 15442) for 5 min and 24 h and colony forming units per millilitre was calculated. The data were analysed using Kruskal–Wallis with Dunn's test (p ≤ 0.05). All tested extracts showed antimicrobial action over the mixed-species biofilms after 24 h. Some extracts eliminated totally the biofilms. The glycolic extract of P. paniculata, H. virginiana, S. barbatiman and G. sylvestre are effective over mixed-species biofilms and may be indicated as endodontic irrigant or intracanal medication. |
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| Keywords: | biofilm: Candida albicans Enterococcus faecalis Pseudomonas aeruginosa Staphylococcus aureus Streptococcus mutans |
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