| 氟对大鼠骨组织Runx2 mRNA及其蛋白表达的影响 |
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| 引用本文： | 梅梅,于燕妮,郭兵.氟对大鼠骨组织Runx2 mRNA及其蛋白表达的影响[J].中国地方病学杂志,2010,29(5). |
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| 作者姓名： | 梅梅 于燕妮 郭兵 |
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| 作者单位： | 贵阳医学院病理学与病理生理学教研室,550004 |
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| 基金项目： | 贵州省科技厅国际合作重点项目，贵州省科技厅社会发展攻关项目，贵州省贵阳市科技局基金资助项目，科技部国际合作项目 |
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| 摘 要： | 目的 观察氟对大鼠骨组织Runx2 mRNA和蛋白表达水平的影响.方法 14只SD大鼠按体质量随机分为对照组饮用自来水,含氟(F-)＜0.06 mg/L]、染氟组(饮水含F-50 mg/L),饲养4个月后股动脉放血处死.采用蛋白质印迹法(Western blot)检测Runx2蛋白表达水平;采用RT-PCR法检测Runx2 mRNA的表达水平.结果 与对照组相比,染氟组大鼠骨组织Runx2 mRNA及蛋白表达水平(2.287±0.261、0.929±0.229)均高于对照组(0.995±0.123、0.317±0.068,t值分别为11.85、6.78,P均＜0.05).结论 氟可导致大鼠骨组织Runx2 mRNA及蛋白表达水平增高,Runx2可能参与了氟引起的骨骼损伤的发生机制.
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| 关 键 词： | 氟化物 基因 蛋白质类 |
Effect of fluoride on expression of runx2 mRNA and protein in bone tissue of rats |
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| MEI Mei,YU Yan-ni,GUO Bing.Effect of fluoride on expression of runx2 mRNA and protein in bone tissue of rats[J].Chinese Jouranl of Endemiology,2010,29(5). |
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| Authors: | MEI Mei YU Yan-ni GUO Bing |
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| Abstract: | Objective To investigate the effect of fluoride on expression of Runx2 mRNA and protein in bone tissue of rats. Methods Fourteen SD rats were randomly divided into two groups: control group(tap water with fluoride ＜ 0.06 mg/L), and fluorosis group(fluoride 50 mg/L in water). After 4 moths, expressions of both mRNA and protein of Runx2 in rat bone tissue were determined by RT-PCR and Western blotting. Results The results showed that the expression of Runx2 mRNA and protein in fluoride-treated bone tissue were 2.287 ± 0.261 and 0.929 ± 0.229, respectively, both of which were significantly higher than those of control group(0.995 ± 0.123,0.317 ± 0.068, t = 11.85,6.78, P ＜ 0.05). Conclusions Fluoride can increase the expression of Runx2 mRNA and protein in bone tissue of rats, and Runx2 may be involved in the pathogenesis of bone injury caused by fluoride. |
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| Keywords: | Runx2 |
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