一期梅毒患者酶毒螺旋体和血清学检测

目的：比较评价几种用于一期梅毒（PS）的实验诊断方法。方法：采用暗视野显微镜法（D－F）、镀银染色法（Ag）、间接免疫荧光法（ⅡF），聚合酶链反应（PCR）等4种梅毒螺旋体（TP）检测方法和甲苯胺红不加红血清学试验（TRUST）和梅毒螺旋体明胶颗粒凝集试验（TPPA）对91例PS患者进行检测。结果：Ag法、ⅡF法、PCR法检测TP阳性率较高，D－F法阳性率较低，对于病程小于2周患者TRUST阳性率明显低于TPPA。结论：Ag法是TP检测的有效方法之一，对于病程小于2周的PS患者TPPA比TRUST更具有诊断价值。

Detection for Treponema Pallidum and Serum Antibodies in Primary Syphilis

Objective To compare and evaluate several methods in diaguosing primary syphilis(PS).Methods 91 PS patients were detected for TP by four kinds of methods:dark field microscopy(D-F),plating argent colouration(Ag),indirect immunofluorescence(IIF) and PCR and serum antibodies by two kinds of serology tests:TRUST and TPPA.Results Positive rate of Ag,IIF and PCR was higher.But that of D-F was lower.To PS patients whose course of disease was shorter than two weeks,positive rate of TRUST significantly was lower than that of TPPA.Conclusion Ag is one of effectual methods for TP.TPPA is more worth using than TRUST to PS patients whose course of disease is shorter than two weeks.