乌司他丁对大鼠肺脏热缺血再灌注损伤的保护作用

 目的 探讨乌司他丁对大鼠肺脏热缺血再灌注损伤的保护作用并比较两种不同剂量的保护效果。方法 30只清洁级SD大鼠，随机分成3组，每组10只。单纯热缺血再灌注组（ischemia-reperfusion group，IR group）阻断左肺门1 h后，开放左肺门再灌注2 h，取左肺静脉血行血气分析后，处死大鼠。左肺上段测湿干比（Wet/Dry Ratio，W/D），中段做组织切片，下段检测MPO浓度；乌司他丁常规剂量组（ulinastatin conventional dose group，UC group）阻断左肺门前，阴茎背静脉注入乌司他丁10 000 IU/kg，其余程序同IR组；乌司他丁大剂量组（ulinastatin high-dose group，UH group）阻断左肺门前，阴茎背静脉注入乌司他丁20 000 IU/kg，其余程序同IR组。结果 IR组与UH组比较，血气分析中肺静脉血的氧分压（PO₂）与W/D的差异均有统计学意义［（109.5±18.3）mmHg vs.（128.5±17.7）mmHg，（5.30±0.13）vs.（5.08±0.25），P<0.05］；MPO浓度检测结果，IR组、UC组与UH每两组间的差异均存在统计学意义［（57.36±10.51）ng/mL，（45.74±8.13）ng/mL与（28.30±6.28）ng/mL，P<0.05］；组织切片可见IR组较UC组和UH组炎性细胞浸润明显。结论 乌司他丁对大鼠肺脏的热缺血再灌注损伤有一定的保护作用，并且存在剂量相关性。

Protection effects of ulinastatin against lung warm ischemia-reperfusion injury in rats

Objective To investigate the protective effect of ulinastatin against lung ischemia-reperfusion injury in rats and to compare the contribution of two different doses. Methods Thirty Sprague Dawley rats were randomly divided into three groups, with 10 rats in each. In ischemia-reperfusion group (IR group), the hilum of left lung was blocked for 1 hour and was then reopened. After 2 hours of reperfusion, blood sample from the left pulmonary vein was taken and the oxygen partial pressure was measured before the rats were sacrificed. The upper part of left lungs was assayed for wet/dry weight ratio (W/D), the middle part was taken for microscopic study, the other parts were frozen in liquid nitrogen for detection of myeloperoxidase concentration (MPO); In ulinastatin conventional dose group (UC group) and ulinastatin high-dose group (UH group), the rats were intravenously administered 10 000 IU/kg and 20 000 IU/kg ulinastatin, respectively, before blocking the hilum of the left lungs. The other procedures were the same as in the IR group. Results Compared IR group with the UH group, there was statistically significant difference in PO2 and W/D ［(109.5±18.3)mmHg vs. (128.5±17.7)mmHg, 5.30±0.13 vs. 5.08±0.25, P<0.05］. For the results of MPO concentration, among IR group, UC group and UH group, there was statistically significant difference between each two groups ［(57.36±10.51)ng/mL, (45.74±8.13)ng/mL and (28.30±6.28)ng/mL, P<0.05］. Tissue slice (HE stain) showed inflammatory cells infiltration in IR group. Conclusions The present study demonstrates that ulinastatin has a protective effect on rat lung warm ischemia-reperfusion injury, which was dose-dependent.