葛根素对大鼠应激性胃黏膜损伤的保护作用

目的:研究葛根素对大鼠应激性胃黏膜损伤的保护作用。方法:采用浸水束缚应激法建立大鼠应激性胃溃疡模型。气囊法记录胃运动,激光多普勒血流计测定胃黏膜血流量,比色法测定胃黏膜组织一氧化氮(NO)和内皮素(ET)含量,并观察胃黏膜组织病理学变化。结果:葛根素可以明显减轻浸水应激引起的胃黏膜损伤;抑制胃运动亢进,尤其是降低胃运动指数、收缩时间百分比和高强度收缩次数;增加胃黏膜血流量;同时增加胃黏膜NO含量,并降低ET含量;胃黏膜组织损害程度显著减轻。结论:葛根素对应激性胃黏膜损伤具有明显的保护作用,其作用机制可能是通过舒张胃黏膜血管,升高胃黏膜NO水平,同时抑制胃运动亢进,增加局部胃黏膜血流量而产生作用。

Protective effect of puerarin on stress-induced gastric mucosal injury in rats

Objective: To study the protective effect of puerarin on stress-induced gastric mucosal injury in rats.Method: The model of gastric ulcer was established by restraint plus water-immersion stress in rats.Gastric motility was monitored by the method of "Gas Balloon".Gastric mucosal blood flow was recorded by laser-Doppler flowmetry.Colorimetric method was used to determine the content of NO and ET in gastric mucosal tissue.Meantime the pathologic changes of gastric mucosal was examined.Result: Puerarin could significantly attenuated gastric mucosal damage induced by water-immersion stress,inhibited gastric motility,specially decreased the index of gastric motility and percentage of gastric contraction time and numbers of violent contraction.The gastric mucosal blood flow and NO level in gastric mucosal were enhanced,while ET level was reduced by puerarin.The degree of tissue damage in gastric mucosal was also significantly attenuated after administration fo puerarin.Conclusion: Puerarin exerts a significant protective effect on water-immersion stress-induced gastric mucosal damage by relaxing the vessels,increasing NO level in gastric mucosal,increasing regional gastric mucosal blood flow and inhibiting gastric motility.