| 结合药效的刺五加纯化工艺优选 |
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| 引用本文： | 谭晓斌,贾晓斌,沈明勤,彭蕴茹. 结合药效的刺五加纯化工艺优选[J]. 中国药房, 2007, 18(12): 902-904 |
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| 作者姓名： | 谭晓斌 贾晓斌 沈明勤 彭蕴茹 |
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| 作者单位： | 1. 江苏省中医药研究院、江苏省现代中药制剂工程技术研究中心,南京市,210028;江苏大学药学院,镇江市,212003
2. 江苏省中医药研究院、江苏省现代中药制剂工程技术研究中心,南京市,210028 |
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| 摘 要： | 目的优选刺五加水提物的纯化工艺。方法将刺五加水提物分别采用D-101大孔树脂、聚酰胺和正丁醇萃取工艺纯化,测定紫丁香苷和总黄酮含量,并比较3种纯化样品对模型大鼠缺血再灌注损伤和模型小鼠断头张口的抗脑缺血作用。结果3种纯化样品均具有抗脑缺血作用,其中D-101大孔树脂纯化样品的作用强于其它2种样品;紫丁香苷含量与刺五加抗脑缺血作用成正相关。结论D-101大孔树脂吸附法是刺五加提取物较佳的纯化工艺。
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| 关 键 词： | 刺五加 紫丁香苷 纯化工艺 D-101大孔树脂 脑缺血 |
| 文章编号： | 1001-0408(2007)12-0902-03 |
| 收稿时间： | 2006-12-04 |
| 修稿时间： | 2007-01-07 |
Pharmacodynamic Optimization of Purification Technique for Acanthopanax Senticosus Harms |
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| TAN Xiaobin,JIA Xiaobin,SHEN Mingqin,PENG Yunru. Pharmacodynamic Optimization of Purification Technique for Acanthopanax Senticosus Harms[J]. China Pharmacy, 2007, 18(12): 902-904 |
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| Authors: | TAN Xiaobin JIA Xiaobin SHEN Mingqin PENG Yunru |
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| Affiliation: | 1.Jiangsu Provincial Academy of Traditional Chinese Medicine, Jiangsu Engineering and Technology Research Center for Modern Chinese Pharmaceutical Preparation, Nanjing 210028,China;2.School of Pharmacy, Jiangsu University, Zhenjiang 212003, China |
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| Abstract: | OBJECTIVE: To optimize the purification technique for the extract of Acanthopanax senticosus Harms. METHODS: The techniques of D- 101 macroporous resin adsorption, polyamide adsorption and n- butanol extraction were separately applied to purify the aqueous extract of Acanthopanax senticosus Harms. Syringin was detected by HPLC and total flavone was detected by UV. The anti- cerebral ischemia effects of three purified samples were compared using rat MCAO model and mouse decapitation gape model. RESULTS: The three purified samples all showed anti- cerebral ischemia effects, with the sample purified via D- 101 macroporous resin adsorption having stronger effects than the other two. The content of Syringin was correlated with anti- cerebral ischemia effects. CONCLUSIONS: D- 101 macroporous resin adsorption is a be-tter technique for purifying the aqueous extract of Acanthopanax senticosus Harms. |
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| Keywords: | Acanthopanax senticosus Harms Syringin Purification technique D-101 macroporous resin Cerebral ischemia |
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