外周血乙型肝炎病毒核酸水平与丙氨酸氨基转移酶关系的探讨

目的　探讨血清中乙型肝炎病毒核酸 (HepatitisBvirus -DNA ,HBV -DNA)含量与丙氨酸氨基转移酶 (Alanineaminotransferase ,ALT)的关系。方法　用荧光实时定量聚合酶链反应 (Fluorescencereal-timequantita tivepolymerasechainreaction ,FQ -PCR)和连续监测法分别检测了 5 2 8例乙型肝炎病毒感染者及非病毒感染者血清中HBV -DNA和ALT水平。结果　当血清中HBV -DNA含量在 10 6拷贝 /毫升以下时 ,随着血清中HBV -DNA含量的增加 ,血清ALT水平升高 ;但当HBV -DNA含量超过 10 6拷贝 /毫升后 ,ALT水平反而随着血清中病毒数量的增加而呈减弱趋势。结论　血清中乙型肝炎病毒数量与ALT之间存在一定的关系 ,但不能只依据血清ALT水平来推断乙型肝炎病毒在体内的复制活跃程度

The relationship of HBV- DNA quantification and ALT for type B hepatitis

Objectives To study the relationship of Hepatitis B virus-DNA(HBV-DNA) quantification and Alanine aminotransferase (ALT) for type B Hepatitis.Methods The concentration of HBV-DNA and ALT were detected by Fluorescence real-time quantitative polymerase chain reaction (FQ-PCR) and successive monitor reaction.The samples were divided into different groups with different orders of magnitude HBV-DNA and Newman-Keuls was used as a statistics methods to check the different of the mean ALT value of each groups.Results The injury of hepatocytes was increased with the step-up of HBV-DNA quantification in serum when the HBV-DNA quantification<10 6 copies/ml, but decreased with the step-up of HBV-DNA quantification in serum when the HBV-DNA quantification>10 6 copies/ml.Conclusions There was some relationship between Hepatitis B virus and ALT, the ALT would decreased with the step-up of HBV-DNA quantification in serum when the HBV-DNA quantification above a liminal value. The ALT level couldn't show the duplication of Hepatitis B virus.