电针“阳陵泉”穴对急性胆囊炎家兔白细胞计数和胆囊壁厚度的影响

目的:观察电针"阳陵泉""阴陵泉"穴对急性胆囊炎家兔白细胞计数、胆囊壁厚度以及组织病理的影响,探讨"合治内府"理论之胆和阳陵泉的相关性。方法:健康成年雄性家兔随机分为正常组、模型组、阳陵泉组、阴陵泉组,每组8只。胆囊内注射大肠杆菌液制成急性胆囊炎模型。两个治疗组电针相应穴位,连续7d。对比治疗前后白细胞计数,B超下观察胆囊壁厚度变化,光镜下观察胆囊组织病理变化。结果:与正常组比较,模型组白细胞计数显著升高,胆囊壁显著增厚(均P0.05);电针后,阳陵泉组、阴陵泉组的白细胞计数较模型组明显降低,胆囊壁显著变薄(均P0.05);与阴陵泉组比较,电针后,阳陵泉组的胆囊壁更薄(P0.05)。病理观察显示,模型组胆囊上皮黏膜变短,有全层坏死改变,胆囊上皮乳头水肿,间质内炎细胞浸润;阳陵泉组基层增生,散在炎细胞浸润;阴陵泉组血管腔内含有成堆的中性粒细胞。结论:阳陵泉穴和阴陵泉穴都能有效地治疗急性胆囊炎。从改善急性胆囊炎家兔的胆囊壁厚度来看,阳陵泉穴比阴陵泉穴效果更明显。

Effect of Electroacupuncture at "Yanglingquan" (GB 34) Acupoint on White Blood Cell Count and Gallbladder Wall Thickness in Rabbits with Acute Cholecystitis

Objective To observe the effects of electroacupuncture(EA)at"Yanglingquan"(GB 34)acupoint on white blood cell(WBC)count and gallbladder wall thickness in rabbits with acute cholecystitis,so as to explore the inherent correlation between the gallbladder and GB 34 acupoint according to the theory of Chinese medicine.Methods Thirty-two male rabbits were randomly and equally divided into control group,model group,EA "Yanglingquan"(GB 34)group(EA-GB 34)and EA "Yinlingquan"(SP 9)group(EA-SP 9).The acute cholecystitis model was induced by intra-gallbladder injection of escherichia coli liquid.EA stimulation was delivered once per day for consecutive 7days.WBC count and gallbladder wall thickness were measured to assess the effects of EA treatment.HE staining was carried out to examine the pathological changes of the gallbladder tissue.Results Compared with the control group,WBC count and gallbladder wall thickness of the model group were significantly increased(P<0.05),and those changes were reversed by EA intervention at either GB 34 or SP 9acupoints(P<0.05).As to WBC count,there was no significant difference between EA-GB 34 and EA-SP 9groups(P>0.05),whereas for reducing gallbladder wall thickness,EA stimulation at GB 34 acupoint resulted in a better effect than that of EA at SP 9acupoint(P<0.05).Conclusion EA produces positive therapeutic benefits on acute cholecystitis in rabbits.It seems that stimulation at"Yanglingquan"acupoint gives rise to a better effect than that of"Yinlingquan"acupoint in particular for the reduction of gallbladder wall thickness.