毛果鱼藤中黄酮类化学成分研究

综合运用硅胶柱色谱、SephadexLH-20、聚酰胺柱色谱、半制备HPLC以及重结晶等方法对毛果鱼藤中黄酮类化学成分进行分离纯化,并通过质谱、核磁共振谱等方法鉴定各单体化合物的结构。最后从毛果鱼藤的乙酸乙酯部位分离得到9个黄酮类化合物,依次为香叶木素(1),3, 3'-二甲醚-槲皮素(2),阿弗洛莫生(3),6, 3'-dihydroxy-7, 4'-dimethoxyisoflavone(4),飞机草素(5),7, 3'-dihydroxy-8, 4'-dimethoxyisoflavone(6),6, 4'-dihydroxy-7, 3'-dimethoxyisoflavone(7),5, 7, 4'-trihydroxy-3, 3', 5'-trimethoxyflavone(8),alpinumisoflavone(9)。所有化合物均为首次从该植物中分离得到。运用MTT法进行体外抗肿瘤实验,结果表明化合物 2 显示一定的抑制细胞增殖活性。

Studies on flavonoids from Derris eriocarpa

Derris eriocarpa, a traditional Chinese medicine belonging to the family of Leguminosae, is widely distributed mainly over Yunnan, Guangxi and Guizhou of China. Modern pharmacological researches on this herb showed that it had extensive bioactivities, such as promoting urination, removing dampness and cough and reducing inspissated mucus and other biological activities. The extensive studies on the chemical constituents of this plant have resulted in the isolation of triterpenoids, steroids, fatty acid and others, but the flavone compounds haven't reported before. In our further research on the ethyl acetate of this plant, nine flavone compounds were obtained by column chromatography on silica gel, Sephadex LH-20, semi-prep HPLC, polyamide column chromatography and recrystallization for separation and purification. The structures were determined on the basis of extensive spectroscopic analysis, including MS, NMR experiments and comparison with spectroscopic data in the literature, respectively, as diosmetin(1), 3, 3'-di-O-methylquercetin(2), afromosin(3), 6, 3'-dihydroxy-7, 4'-dimethoxyisoflavone(4), odoratin(5), 7, 3'-dihydroxy-8, 4'-dimethoxyisoflavone(6), 6, 4'-dihydroxy-7, 3'-dimethoxyisoflavone(7), 5, 7, 4'-trihydroxy-3, 3', 5'-trimethoxyflavone(8), and alpinumisoflavone(9). All these compounds were isolated from Derris eriocarpa How for the first time. And the in vitro assays showed that compound 2 possessed moderate inhibitory activity against human cancer cells K562 and HEL.