| 大鼠局灶性脑缺血后星形胶质细胞Cyclin D1及Cyclin E的表达 |
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| 引用本文： | 席青松,朱丽霞,王萍,王晶,陈华先,王芳,张琼,王伟. 大鼠局灶性脑缺血后星形胶质细胞Cyclin D1及Cyclin E的表达[J]. 神经损伤与功能重建, 2010, 5(1): 14-16 |
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| 作者姓名： | 席青松 朱丽霞 王萍 王晶 陈华先 王芳 张琼 王伟 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院,肿瘤科,武汉,430030;华中科技大学同济医学院附属同济医院,妇产科,武汉,430030;华中科技大学同济医学院附属同济医院,神经科,武汉,430030;华中科技大学同济医学院附属同济医院,神经科,武汉,430030 |
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| 基金项目： | 国家杰出青年基金(No.30725019) |
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| 摘 要： | 目的:研究大鼠局灶性脑缺血后星形胶质细胞细胞周期蛋白Cyclin D1及Cyclin E的表达差异及变化。方法:建立大鼠大脑中动脉闭塞模型,双光源流式细胞术检测假手术组及缺血再灌注后1d、3d、7d、15d组大鼠大脑皮质星形胶质细胞Cyclin D1及Cyclin E的表达。结果:假手术组大鼠星形胶质细胞表达Cyclin D1及Cyc-lin E,Cyclin E的表达高于Cyclin D1(P0.05);局灶性脑缺血后,星形胶质细胞Cyclin D1的表达逐渐增加,7d及15d时高于假手术组(P0.05);局灶性脑缺血后,星形胶质细胞Cyclin E的表达无明显变化,各组间比较差异无统计学意义。结论:局灶性脑缺血后星形胶质细胞Cyclin D1表达增强,提示Cyclin D1可能参与脑缺血后星形胶质细胞的活化增殖。
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| 关 键 词： | Cyclin D1 Cyclin E 脑缺血 流式细胞术 星形胶质细胞 |
Expressions of Cyclin D1 and Cyclin E in Astrocytes of Rats after Focal Cerebral Ischemia |
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| XI Qing-song,ZHU Li-xia,WANG Ping,WANG Jing,CHEN Hua-xian,WANG Fang,ZHANG Qiong,WANG Wei. Expressions of Cyclin D1 and Cyclin E in Astrocytes of Rats after Focal Cerebral Ischemia[J]. Neural Injury and Functional Reconstruction, 2010, 5(1): 14-16 |
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| Authors: | XI Qing-song ZHU Li-xia WANG Ping WANG Jing CHEN Hua-xian WANG Fang ZHANG Qiong WANG Wei |
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| Abstract: | Objective:To investigate the change of the expression of Cyclin D1 and Cyclin E in astrocytes of rats after focal cerebral ischemia.Methods:Ischemia was induced by temporary middle cerebral artery occlusion(MCAO).The rats suffered from MCAO were sacrificed on days 1,3,7 or 15 after reperfusion and rats in control group were sacrificed on day 1 after surgical operation.The brain was taken and the expressions of Cyclin D1 and Cyclin E in astrocytes were detected by flow cytometry.Results:The expressions of Cy... |
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| Keywords: | Cyclin D1 Cyclin E cerebral ischemia flow cytometry astrocytes |
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