白藜芦醇抑制SHG-44胶质瘤细胞生长实验研究

目的探讨白藜芦醇(Res)在体外诱导脑胶质瘤SHG-44细胞凋亡并抑制其生长的作用。方法四甲基偶氮唑蓝(MTT)比色法测量不同剂量的Res作用6h、24h和48h后对SHG-44胶质瘤细胞增殖的影响。HE染色、Hoechst33342荧光染色观察细胞形态改变,DNAladder检测细胞DNA裂解情况,流式细胞仪用异硫氰酸荧光素标记的膜联蛋白V(AnnexinV-FITC)和碘化丙啶(PI)双染检测凋亡率,并测定细胞周期的改变。结果Res明显抑制SHG-44细胞的生长和增殖(P<0.01),呈浓度及时间依赖性反应;Res所致的SHG-44胶质瘤细胞凋亡为浓度依赖关系,随着浓度的增高,凋亡更明显。此凋亡细胞周期主要发生G1期比例升高,S、G2期比例降低。结论Res明显抑制SHG-44细胞生长并诱导其发生凋亡和细胞周期改变,为Res用于治疗脑胶质细胞瘤提供了实验依据。

Experimental study on resveratrol suppressing the growth of SHG-44 glioma cells

Objective To explore the contribution of resveratrol (Res) to the proliferation and apoptosis of brain glioma SHG-44 cells in vitro.Methods Methyl thiazolyl tetrazolium(MTT)assay was used to measure its effects on the proliferation of SHG-44 cells cultured with different concentrations of Res for 6 h, 24 h, and 48 h. HE staining and Hoechst 33342 fluorescence staining were used to observe the cellular morphological changes. DNA ladder assay was used to detect the schizolysis of cellular DNA, the Annexin V-fluorescein isothiocyanate (FITC)and propidium iodide(PI) assay by flow cytometer(FCM) were applied to detect the apoptosis induced by Res. The changes of cell cycle were detected by FCM.Results Res obviously suppressed the proliferation of SHG-44 glioma cells (P<0.01) in a concentration- and time-dependent manner. Res induced cellular apoptosis of the SHG-44 cells in a concentration-dependent manner, and mainly with an accumulation of G_ 1 and a reduction in the G_ 2 , S phase of the cell cycle.Conclusion Resveratrol significantly suppresses the growth of glioma cells, induces the apoptosis and changes the cell cycle of SHG-44 glioma cells. Therefore resveratrol may be considered as a possible treatment medicine for gliomas.