| 硫酸皮肤素对大鼠血管纤溶酶原活化素释放的影响 |
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| 引用本文： | 朱广瑾,Abbadini M,Maggi A.硫酸皮肤素对大鼠血管纤溶酶原活化素释放的影响[J].中国医学科学院学报,1988,10(6):435-438. |
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| 作者姓名： | 朱广瑾 Abbadini M Maggi A |
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| 作者单位： | 中国医学科学院基础医学研究所，意大利Mario Negri药理学研究所，英国伦敦药理学院 北京 |
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| 摘 要： | 用大鼠下肢血管床灌流模型,观察肝素样药物对纤维蛋白溶解系统的作用。结果表明,硫酸皮肤素(DS)能诱导大鼠肢体血管壁释放纤溶酶原活化素(PA)。DS浓度0.1～0.8mg/ml时,给药后1～2min内PA活性增高达最大值。DS在0.1～0.4mg/ml范围内时PA释放与DS浓度成正比,DS0.4mg/ml可诱导最大的PA释放反应。SDS-聚丙烯酰胺凝胶电泳和纤维蛋白自显影分析证实,DS诱导的是组织类型纤溶酶原活化素(t-PA),其分子量为67000。
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| 关 键 词： | 硫酸皮肤素 纤溶酶原活化素 组织类型纤溶酶原活化素 纤维蛋白自显影 |
Effect of Dermatan Sulphate on Plasminogen Activator Activity in Perfused Rat Hindlegs |
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| Zhu Guangjin,et al.Effect of Dermatan Sulphate on Plasminogen Activator Activity in Perfused Rat Hindlegs[J].Acta Academiae Medicinae Sinicae,1988,10(6):435-438. |
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| Authors: | Zhu Guangjin |
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| Abstract: | Effect of dermatan sulphate (DS) on plasminogen activator (PA) activity was studied in rat perfusion model. Perfusion of different doses of DS (0.1-0.8 mg/ml) resulted a increase of PA activity which reached the maximum within the 1-2 min after perfusion, gradual increase of PA activity being noticed within a certain concentration range of DS (0.1-0.4 mg/ml) and decrease thereafter (0.6-0.8mg/ml). The type of PA activity increase during DS perfusion was characterized by SDS-PAGE and fibrinautography as tissue -type PA (t-PA) on the basis of its molecular weight (67 000) which could be inhibited by a specific anti-t-PA antiserum. |
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| Keywords: | dermatan sulphate plasminogen activator tissue-type plasminogen activator fibrinautography |
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