鼻咽癌发生发展过程中EBER1表达的研究

目的：探讨ＥＢＶ与鼻咽癌发生发展的关系及其病理学意义。方法：应用原位杂交技术检测５０例鼻咽癌石蜡切片组织中ＥＢＥＲ１的表达及分布状况。结果：正常鼻咽粘膜上皮及其单纯性增生、鳞状化生上皮ＥＢＥＲ１表达均阴性，鼻咽癌组织中表达率为９８％（４９／５０），且在转移灶中不丢失。鼻咽异型增生上皮亦可表达ＥＢＥＲ１阳性信号（１６／２０），结论：ＥＢＶ与鼻咽癌关系密切，ＥＢＶ可能在鼻咽上皮的恶性转化过程中起重要作用，鼻咽上皮的异型性改变尤其是ＥＢＥＲ１阳性者可认为是癌前病变，随访监测可能有利于早期发现鼻咽癌。

QUANTITATIVE STUDY ON EBER 1 EXPRESSION DURING THE DEVELOPMENT OF NASOPHARYNGEAL CARCINOMA

Objective:To study the relationship between Epstein Barr virus(EBV)and development of nasopharyngeal carcinoma(NPC)as well as pathological significance of EBV encoded small RNA A(EBER 1) expression.Methods:The expression and distribution of EBER 1 in paraffin embedded tissue from 50 cases of NPC were studied by in situ hybridization.Results:EBER 1 was not detected in normal and simple hyperplasia of nasopharyngeal epithelial cells and metaplasia of nasopharyngeal mucosal epithelia.Of the 50 cases of NPC,49 cases(98%)showed the expression of EBER 1 that was still present in metastatic NPC cells and dysplastic cells of paratumourous nasopharyngeal epithelium.Conclusion:There is a strong association between EBV and NPC,and EBV may play an important role in the pathogenesis of NPC.The dysplastic cells of nasopharyngeal epithelium especially those positive for EBER 1 can be regarded as a kind of precarcinomatous change.The measurement of EBER 1 may be helpful in screening early NPC in high risk populations.