HPLC法测定麝香草酚醇溶液中麝香草酚的含量

目的：建立 HPLC 法测定麝香草酚醇溶液中麝香草酚的含量。方法：采用 Agilent Eclipse XDB-C18（250 mm ×4.6 mm,5μm）色谱柱,甲醇-水（65∶35）为流动相,流速为1.0 ml·min^-1,检测波长为275 nm,进样量为10μl,柱温为25℃。结果：麝香草酚在60~160μg·m^l-1范围内与其峰面积线性关系良好（r=0.9995）,平均回收率为101.59%（n=9）,RSD值为1.39%。结论：本方法简便快捷、准确度高、专属性强、灵敏度高,可用于麝香草酚醇溶液中麝香草酚的含量测定。

Determination of Thymol in Thymol Alcoholic Solutions by HPLC

Objective：To establish an HPLC method for the determination of thymol in thymol alcoholic solutions. Methods： An Agilent Eclipse XDB-C18（250 mm ×4.6 mm,5μm） column was used with the mobile phase of methanol-water （65∶35）, the flow rate was 1. 0 ml·min^-1 . the detection wavelength was 275nm, the injection volume was 10μl, and the column tenperature was 25℃. Re-sults：A good linear correlation of thymol was observed within the range of 60-160 μg·ml^-1（r=0. 999 5）. The average recovery was 101. 59% with RSD of 1. 39%（n=9）. Conclusion：The method is quick, simple and accurate, which can be used in the determina-tion of thymol alcoholic solutions with good selectivity and sensitivity.