肾上腺髓质素放射免疫分析法的建立及应用研究

目的：建立肾上腺髓质素（ADM）放射免疫分析法（RIA），探讨人及动脉心血管生理病理时ADM的变化。方法：制备特异性抗ADM血清，用氯胺－T法制备125I－ADM，建立了灵敏的ADM RIA，对正常人，冠心病，高血压患者进行血浆ADM检测，同时观察125I－ADM于大鼠尾静脉注射6h后在各脏器中的分布， 血管内皮细胞和平滑肌细胞12，24，36，48和72h细胞培养上清液的ADM含量。结果：该方法的灵敏度为2．32ng/L。高血压，冠心病患者ADM明显高于对照组，125I0ADM在鼠各脏器的分布以肾脏最高，肺，血管内皮次之，平滑肌最低，鼠血管内皮细胞和平滑肌细胞上清细胞培养ADM含量峰值在24h，分别为46．54ng／L和6．71ng/L。结论：ADM RIA可为心血管疾病的研究，诊断和治疗提供依据。

A study on adrenomedullin radioimmunoassay kit and its clinical applications

Objective To find a radioimmunoassay(RIA) method of adrenomedullin (ADM) and its clinical applications. Methods The serum ADM antibody was prepared. ADM was labelled by the chloramine-T method. Concentration of serum ADM of coronary heart disease, hypertension patients and normol subjects was measured with this RIA method. The uptakes of 125 I-ADM of different tissues of mice were measured six hours after intravenous 125 I-ADM administration. Results The results showed that the concentrations of serum ADM in coronary heart disease, hypertension patients was significantly higher than that in normol subjects.From higher to lower,the order of 125 I-ADM uptakes in different tissues was as follows: kidney,lung, angioendothelial, pancreatic,brain,and smooth muscle tissue.The concentration of ADM in the supernatants of mouse angioendotheliocyte and smooth muscle cell cultures at different intervals were 46.54 ng/L and 6.71 ng/L and the highest value is at 24 h. Conclusions RIA-ADM can offer a reliable method for study,diagnosis and treatment of coronary heart disease,hypertension in clinical practising.