基质金属蛋白酶-9与糖尿病肾病的相关性

目的观察大鼠糖尿病肾病(DN)发展过程中肾脏局部基质金属蛋白酶-9(MMP-9)表达的改变,分析其与DN的相关性。方法对16只链脲佐菌素(STZ)糖尿病大鼠模型分别于造模后第4、8周通过免疫组织化学染色法观察肾脏局部MMP-9的表达,以MMP-9平均积分吸光度(IDP)为观察指标。结果正常对照组第4、8周大鼠肾脏MMP-9表达(IDP分别为15.6±73.4,16.2±3.1)呈强阳性;实验组第4、8周大鼠肾脏MMP-9的表达(IDP分别为8.2±0.9,3.9±0.6)呈递减(P<0.01)。结论MMP-9表达减少是DN早期病理改变的重要原因之一。

Correlation between matrix metalloproteinase-9 and diabetic nephropathy

Objective To observe the changes of the expression of metalloproteinase(MMP)-9 at kidney with the development of diabetic nephropathy(DN) in streptozotocin(STZ)-induced diabetic rats and to investigate their correlation.Methods Sixteen female Wistar rats were used to induce diabetes mellitus by intraperitoneal injection of STZ(60 mg/kg).Immunohistochemistry was used to detect MMP-9 expressions in kidneys with image analysis system on the 4th and 8th weeks.Results In this experiment,the expression of MMP-9 in diabetic kidneys on the 4th and 8th weeks(8.2±0.9,3.9±0.6) were both significantly lower than that in normal control rats(15.6±73.4,16.2±3.1,P<0.01),and the level on the at 8th week (3.9±0.6) was lower than that on the 4th week(8.2±0.9,P<0.01).Conclusion The reduced expression of MMP-9 is one of the main reasons for impairing renal structure of DN.