| 抗淋球菌IgA蛋白酶单克隆抗体特性的初步研究 |
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| 引用本文： | 白光春 孙怡群. 抗淋球菌IgA蛋白酶单克隆抗体特性的初步研究[J]. 细胞与分子免疫学杂志, 1997, 13(3): 48-49,59 |
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| 作者姓名： | 白光春 孙怡群 |
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| 作者单位： | [1]第四军医大学 [2]西京医院检验科 |
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| 摘 要： | 本文对以重组淋球菌ＩｇＡ蛋白酶为抗原制备的７株特异性ＭｃＡｂ的特性进行了初步研究。结果有５株（１Ｃ８、１Ｅ５、１Ｆ１１、１Ｇ３和２Ｅ６）能中和淋球菌ＩｇＡ蛋白酶活性。经相加试验初步证明，其中１Ｆ１１、２Ｇ３与其它３株ＭｃＡｂ的作用位点不同。因此，淋球菌ＩｇＡ蛋白酶至少存在３个中和表位。
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| 关 键 词： | 淋球菌 IgA蛋白酶 单克隆抗体 |
Preliminary Characterization of McAbs against IgA Protease of Neisseria gonorrhoeae |
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| Bai Guangchun,Li Yuan,Xu Fuming,et al.. Preliminary Characterization of McAbs against IgA Protease of Neisseria gonorrhoeae[J]. Chinese journal of cellular and molecular immunology, 1997, 13(3): 48-49,59 |
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| Authors: | Bai Guangchun Li Yuan Xu Fuming et al. |
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| Abstract: | Seven monoclonal antibodies(McAbs)against IgA protease of Neisseria gonorrhoeae were raised by hybridoma technique The titers of ascite fluid secreted by seven cell lines ranged from 10 -8 to 10 -6 Five McAbs were identified capable of inhibiting IgA protease activity secreted by Neisseria gonorrhoeae, and they were named 1C8, 1E5, 1F11, 1G3, and 2E6 According to the adding test of McAbs, 1C8, 1E5 and 2E6 shared the same epitope, while 1F11 and 1G3 recognized other two epitopes The results suggested that at least three epitopes were present in IgA protease of Neisseria gonorrhoeae The neutralizing McAbs that we had prepared may be useful for further study of IgA protease |
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| Keywords: | Neisseria gonorrhoeae IgA protease monoclonal antibody |
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