病毒性L6565白血病细胞克隆株的建立及其特性研究进展

Ｔ638和Ｌ6565模型是目前国内仅有的两株ＲＮＡ病毒诱发的白血病 ,均属于我国天津 (Ｔ) -上海 (Ｓ) -遵义 (Ｚ)小鼠白血病系统之内[1 ,2 ] 。近年来未见Ｔ638白血病研究的进一步报道 ,情况不详。Ｌ6565病毒性淋巴细胞白血病是由程立等于 1 965年建立的瘤株[3～ 6] 。 1 986年张雷等[7] 应用Ｌ6565白血病小鼠外周血液无细胞提取液感染ＮＩＨ/3Ｔ3细胞 ,在体外建成了Ｌ6565-Ｂ1 细胞系 ,惜已失传。最近 ,殷莲华等[8] 将Ｌ6565白血病小鼠脾脏在灭菌条件下制成细胞悬液后 ,用ＲＰＭＩ 1 640液和 1 0 %小牛血清中培养 2 0代后 ,再用有限稀释法置…

Progress in the study on establishment and characteristics of viral L6565 cell clone

The biological characteristics of viral L6565 leukemia cell clone were as follows: (1) The chromosome counts varied 38～114 , and stem cells were 42; (2) Virus particles type A and type C found in the cytoplasm of clone cells; (3) X-C assays were positive, c-myc and c-fos gene overexpressed in clone cells; (4) Differential markers CD4, CD8, CD45R were negative, CD45RO λ were positive; (5) The supernatant of clone cells could induce T or B lymphocytic leukemia/lymphoma and granulocytic leukemia in SSB strain mice. The leukemogenic effect of concentrate supernatant was stronger than non-concentrate supernatant (P<0.05); (6) The antisense of the c-myc gene induced low expression of c-myc protein, and inhibited the growth of viral L6565 clone cells.