肝郁-心理应激对子宫肌瘤大鼠性激素及免疫因子影响的实验研究

目的:探讨肝郁-心理应激在ULM发病机制中的重要作用,为临床从肝郁-心理应激角度防治ULM提供理论依据,并为中医学"情志学说"提供新的理论依据。方法:将80只SD雌性大鼠按完全随机设计分为正ULM组大鼠建立慢性束缚应激模型,ULM模型组及肝郁+ULM组大鼠建立子宫肌瘤模型,16周后处死全部大鼠,IL-6、INF-γ、TNF-α等细胞因子的含量。结果:肝郁-心理应激可明显增加大鼠子宫重量及系数(P<0.05或P<0.01),降低血清E2、P含量(P<0.05或P<0.01),升高血清LH、FSH含量(P<0.05或P<0.01),并可使胸腺指数明显降低(P<0.01),降低血清IL-2、INF-γ含量(P<0.05或P<0.01),升高血清IL-6、TNF-α含量(P<0.05)。结论:肝观察各组大鼠一般行为学改变,测量子宫、胸腺、脾脏湿重,并计算各脏器指数,HE染色后观测各组大鼠子宫病理组织学变化,双抗体夹心酶联免疫吸附技术(ELISA)测定大鼠血清中E2、P、FSH、LH等性激素含量及血清中IL-2、

Experimental Study of Effect of Liver Qi Stagnation-psychological Stress on Sex hormone and Immune Factors in Uterine Leiomyomas Animal Model

Objective:Discuss the role of Liver qi stagnation-psychological which was played in the pathogenesis of uterine leiomyomas,provide the oretical basis of clinical therapy of uterine leiomyomas from the liver qi stagnation-psychological stress view and a new theory in Chinese medicineemotions theory.Methods:A total of 80 Kunming mice were randomized into normal control group,ULM group,liver qi stagnation-psychological stress group,liver qi stagnation-psychological stress-uterine leiomyomas group;each group co...