宫颈癌组织中微小RNA 155水平及其对细胞增殖和侵袭的影响#br##br#

目的探讨微小RNA 155（miR 155）在宫颈癌组织中的表达及其对宫颈癌细胞增殖和侵袭的影响。方法收集本院2014年5月至2016年12月经手术切除的宫颈癌组织76例、正常宫颈组织63例及宫颈上皮内瘤样病变（CIN）组织84例（CINⅠ级22例、CIN Ⅱ级29例和CIN Ⅲ级33例），采用实时定量PCR（QPCR）检测以上组织中的miR 155水平，分析miR 155水平与宫颈癌临床病理特征（年龄、FIGO分期、肿瘤大小、组织学分级、病理类型、血管侵犯和淋巴结转移）的关系；向宫颈癌HeLa细胞分别转染miR 155抑制物（转染组）和阴性对照（对照组），采用QPCR检测转染48 h后各组的miR 155水平，分别采用MTT法和Transwell侵袭实验检测增殖率和穿膜细胞数目。结果QPCR检测发现宫颈癌组织中的miR 155水平为4270±1901，高于正常宫颈组织和CIN组织（P＜005）；miR 155水平与宫颈癌患者的年龄、组织学分级和病理类型均无关（P＞005），但与FIGO分期、肿瘤大小、血管侵犯和淋巴结转移有关（P＜005）。转染miR 155抑制物48 h后，转染组HeLa细胞的miR 155水平为0265±0034，低于对照组的1027±0158，差异有统计学意义（P＜005）；与对照组相比，转染组的增殖率和穿膜细胞数均降低，差异有统计学意义（P＜005）。结论miR 155在宫颈癌组织中呈高表达，且与临床分期、血管侵犯和淋巴结转移有关，参与宫颈癌的发生发展。降低miR 155水平可抑制增殖和侵袭过程，在宫颈癌的防治中有一定价值。

Impact of colorectal carcinoma cell line biological behaviour by lobaplatin and oxaliplatin

Objective:To observe the biological behaviour effect of colorectal carcinoma cell line by lobaplatin and oxaliplatin.Methods:The human colorectal carcinoma cell line LoVo and HR-8348 were used as the research object,and different density lobaplatin and oxaliplatin were as inhibitor.The invasion and metastasis potential were evaluated with transwell chamber.The cell inhibition ratio was measured with MTT assay.Results:Lobaplatin and oxaliplatin could make that the abilities of invasion and metastasis were dr...