甲状腺乳头状癌组织中炎性因子的表达及其临床意义

目的：探讨甲状腺乳头状癌组织中炎性因子的表达及其与甲状腺乳头状癌临床病理特征的关系，为指导临床用药和判断预后奠定实验基础。方法：选取穿刺确诊为甲状腺乳头状癌的74例住院患者作为实验组，另选同期26名体检健康者作为正常对照组。2组受试者均空腹抽取静脉血，实验组患者于术后留取癌组织、癌旁正常组织（距癌组织2 cm）及术后7 d再次空腹抽取静脉血。采用酶联免疫法检测血清、癌旁正常组织及癌组织中白细胞介素1α（IL-1α）、IL-1β和环氧合酶-2（COX-2）蛋白的表达水平；采用实时荧光定量法和免疫组织化学方法检测甲状腺乳头状癌组织及癌旁正常组织中IL-1α、IL-1β和COX-2的mRNA和蛋白
表达水平；分析IL-1α、IL-1β和COX-2表达与甲状腺乳头状癌的临床分期、病理类型及有无淋巴结转移的关系。结果：实验组患者血清中IL-1α、IL-1β和COX-2的蛋白表达水平明显高于正常对照组（P<0.01）；甲状腺乳头状癌患者癌组织中IL-1α、IL-1β和COX-2的蛋白表达水平、蛋白阳性表达强度及mRNA表达水平明显高于癌旁正常组织（P<0.01）。在甲状腺乳头状癌组织中IL-1α与IL-1β、IL-1α与COX-2、IL-1β与COX-2均呈正相关关系（r=0.64,P=0.035;r=
0.71,P=0.042;r=0.69,P=0.038)。结论：甲状腺乳头状癌组织中IL-1α、IL-1β和COX-2表达水平上调，提示炎性因子参与甲状腺乳头状癌浸润和转移，其应用有望成为新的肿瘤治疗策略和手段。

Expressions of inflammatory factors in thyroid papillary carcinoma tissue and their clinical significances

Objective To investigate the expressions of inflammatory factors in papillary thyroid carcinoma tissue and their relationships with the clinical pathological characteristics,and to provide an experimental basis for guiding clinical treatment and prognosis.Methods 74 patients with papillary thyroid carcinoma dignosed by puncture were selected as experimental group,and 26 cases of healthy people were used as normal control group.The fasting venous blood samples from the subjects in two groups were collected,and the postoperative specimens from cancer tissue,adjacent normal tissue and the fasting venous blood 7dafter operation of the patients in experimental group were collected again.The interleukin 1α(IL-1α),interleukin 1β(IL-1β)and cyclooxygenase-2(COX-2)protein expression levels in serum,cancer adjacent normal tissue and cancer tissue were detected by ELISA;the gene and protein expression levels of IL-1α,IL-1βand COX-2were examined by real-time fluorescent quantitative method and immunohistochemistry;the relationships between their expressions and clinical stages,pathological types,lymph node metastasis of thyroid papillary carcinoma were analyzed.Results The expression levels of serum IL-1α,IL-1βand COX-2protein of the patients in experimental group were significantly higher than those in normal control group(P<0.01);the gene and protein expression levels of IL-1α,IL-1βand COX-2in thyroid papillary carcinoma tissue were significantly higher than those in adjacent tissue(P<0.01).There were positive correlations between the expressions of IL-1αand IL-1β,IL-1αand COX-2,IL-1βand COX-2in thyroid papillary carcinoma tissue(r=0.64,P=0.035;r=0.71,P=0.042;r=0.69,P=0.038).Conclusion The expression levels of IL-1α,IL-1βand COX-2in thyroid papillary carcinoma tissue are increased,suggesting that inflammation is involved in metastasis and invasion of thyroid papillary carcinoma,its application is expected to become a new means of cancer treatment strategies.