国产蛇毒激活血浆蛋白C的研究

目的:从9种国产蛇毒中筛选具有激活血浆蛋白C作用的蛇毒。方法:运用活化部分凝血活酶时间(APTT)和发色底物实验分别观察抗凝活性和酰胺酶活性,综合抗凝活性和酰胺酶活性确定蛋白C蛇毒激活作用。结果:在9种国产蛇毒中烙铁头蛇毒及蝮蛇毒在1.5mg/L浓度下即使人血浆纯蛋白C产生酰胺酶活性,并使APTT显著延长。结论:9种国产蛇毒中烙铁头蛇毒及蝮蛇毒具有激活人体血浆蛋白C成为活化蛋白C(APC)的作用。

Identification of protein c activator from nine species of Chinese snake venoms

AIM:To determine which species of snake venoms contained protein c activator among 9 species of Chinese snake venoms. METHODS: Anticoagulant activity was examined by activated partial thromboplastin time (APTT) assay,and amidolytic activity was measured with activated protein c (APC) specific chromogenic peptide substrate-chromozy APC. RESULTS: Among 9 species of Chinese snake venoms,Trimeresurus mucrosquamatus venom and Agkistrodon halys venom were not only able to generate amidolytic activity from purified human PC, but also prolonged APTT strongly even at such a concentration as 1.5 mg/L.CONCLUSION: Trimeresurus mucrosquamatus venom and Agkistrodon halys venom contain protein c activator which activating human plasma PC into APC.