布洛芬对油酸型急性肺损伤防治作用的实验研究

目的 :探讨布洛芬预防给药防治急性肺损伤 (ALI)的疗效及其可能机制。方法 :用油酸复制ALI大鼠模型。实验分正常对照组、模型组和布洛芬治疗组 ,检测各组肿瘤坏死因子 α(TNF α)浓度、肺湿干比重、肺系数 ,并对肺组织进行病理组织学检查。结果 :布洛芬治疗组在 1.5h时间点血浆TNF α浓度 ( 3 71.0 0± 41.60 pg/ml)较之模型组 ( 73 3 .2 0± 2 48.5 0pg/ml)有显著性降低 (P <0 .0 5 ) ,肺湿干重比、肺系数分别减至 5 .80± 1.0 0和 5 .40± 0 .70 ,肺组织损伤明显减轻。结论 :布洛芬能减少TNF α的产生 ;布洛芬预处理可能是减轻ALI的原因之一。

Experimental Study of Prophylaxis Against the Oleic Acid Induced Acute Lung Injuries by Ibuprofen

Objective: To explore the effect and its probable mechanism of Ibuprofen treating Acute Lung Injury(ALI).Method: To copy the model of ALI with oleic acid. Adult Wistar rats were divided into three groups:normal control group,oleic acid group,Ibuprofen pretreating group. The concentration of plasma TNF in different groups and in different time were measured by ELISA,and the ratio of lung wet weight to dry weight,lung coefficient and histopathologic examination which served as indexes of lung injury was calculated and observed.Results: Compared with oleic acid group at 1.5hr time point,the level of plasma TNFα in Rhubarb group decreased significantly( P <0.05); the ratio of lung wet weight to dry weight and lung coefficient decreased to 5.80±1.00( P <0.01,vs oleic acid group) and 5.40±0.70( P <0.01,vs oleic acid group); histopathologic examination showed the degree of ALI relieved apparently in pretreating group.Conclusion: Using Ibuprofen may contribute to prevent lung injury in rats,this protective effect may at least partly due to the inhibition of TNF.