灵芝孢子粉对无镁活化海马神经元NGF的影响

目的：本实验研究灵芝孢子粉对体外无镁活化海马神经元NGF（nerve growth factor神经生长因子的影响,进一步研究癫痫的发病机制及灵芝孢子粉的作用机制。方法：采用新生Wistar大鼠（出生后24h之内）,分离海马神经元,进行体外原代培养。运用Western-blot检测不同时间点各组细胞NGF的表达水平。结果：NGF五个时间点表达强度与模型组中相应的时间点比较增强明显减弱（均P〈0.05）。结论：NGF的阳性表达被完全抑制,灵芝孢子粉能有效减少癫痫对海马神经元细胞所造成的损害。

Effect of Ganoderma lucidum spores on magnesium-free activated hippocampal neurons NGF

Objective：To investigate the effect of ganoderma lucidum spores on the change of NGF after epileptiform discharge of hippocampal neurons with magnesium-free extracellular fluid to further study epilepsy mechanism and the anti-epilepsy mechanism of ganoderma lucidum spores.Methods：Neonatal Wistar mice were killed to get hippocampal neurons which were cultured.By using Western-blotting,we observed the changes in the NGF expression.Results：NGF was reduced across all five time points（P〈0.05）.Conclusion：NGF expression is inhibited completely,and Ganoderma lucidum spores can prevent the damages of epilepsy to hippocampal nerve cells.