肝硬变及肝癌癌旁肝组织中小多角肝细胞性质的免疫组化研究

应用免疫组织化学的方法对６０例ＨＣＣ、癌旁肝组织和４７例肝硬变组织中ＨＢｘＡｇ和ＩＧＦ Ⅱ表达进行实验观察，以探讨小多角肝细胞（ＳＰＬＣ）的性质。结果显示，在癌旁肝和肝硬变的ＳＰＬＣ中ＨＢｘＡｇ和ＩＧＦ Ⅱ的阳性表达率分别为６９１４％和６６６７％，其中ＨＢｘＡｇ阳性的ＳＰＬＣ中ＩＧＦ Ⅱ阳性率为８２１４％，而ＨＢｘＡｇ阴性的ＳＰＬＣ中ＩＧＦ Ⅱ阳性率为３２００％。ＩＧＦ Ⅱ在ＨＢｘＡｇ阳性的ＳＰＬＣ中的表达明显高于ＨＢｘＡｇ阴性ＳＰＬＣ组（Ｐ＜００５）。结果表明，ＳＰＬＣ为癌旁肝和肝硬变等病变肝组织中一种幼稚的肝细胞成分，具有肝细胞和胆管细胞分化潜能，其增殖和分化的作用可能与肝细胞再生、胆管增生关系密切

Characterizations of the Small Polygonal Liver Cell in Human Liver Cirrhosis and Tissues Surrounding Hepatocellular Carcinoma

In order to investigate the characterizations of the small polygonal liver cell (SPLC) in cirrhotic liver tissues and the liver tissues surrounding the tumors, 60 cases of hepatocellular carcinoma (HCC) and 47 cases of liver cirrhosis (LC) were examined with immunocytochemistry method using antibodies against IGF Ⅱ and HBxAg.The results showed that HBxAg and IGF Ⅱ positive rates were 69 14% and 66 67% respectively on SPLC in pericancerous liver tissues and LC, the positive rate of IGF Ⅱ were 82 14% in HBxAg positive SPLC group but 32 00% in HBxAg negative SPLC group.The expression of IGF Ⅱ in HBxAg positive SPLC was greater than that in HBxAg negative SPLC (χ 2 test, P <0 05).The above findings suggest that SPLC might be a kind of stem like cells and there were closely relations between proliferation and differentiation of SPLC and regeneration hepatocytes and biliary tuctular cells.