| 经颅磁刺激对脑出血大鼠脑细胞增殖的影响 |
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| 引用本文： | 卢旺盛,田增民,郭欣茹,霍小林,李玥,刘爽,王亚明,尹丰.经颅磁刺激对脑出血大鼠脑细胞增殖的影响[J].中国脑血管病杂志,2008,5(12). |
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| 作者姓名： | 卢旺盛 田增民 郭欣茹 霍小林 李玥 刘爽 王亚明 尹丰 |
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| 作者单位： | 1. 海军总医院全军神经外科中心,北京,100037
2. 中国科学院电工研究所生物电磁研究组 |
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| 摘 要： | 目的观察经颅磁刺激对脑出血大鼠神经功能恢复的作用,及对脑细胞增殖的影响。方法健康雄性SD大鼠42只,随机取12只作为对照组,30只大鼠采用尾状核注射Ⅶ型胶原酶法制作脑出血模型。剔除死亡大鼠6只。随机将24只大鼠分为模型组和经颅磁刺激组,每组12只。经颅磁刺激采用频率为0.5Hz,脉冲刺激波宽为72μs,刺激强度为阈上刺激1.44T,1次/d,60个脉冲/次,连续刺激7d。采用Longa法分别于脑出血后1、7、14、21及28d对各组大鼠进行神经功能缺损评分;应用腹腔注射5-溴脱氧尿嘧啶核苷(BrdU)标记增殖细胞。于7、14和28d,应用免疫组化方法检测大鼠脑出血周围组织的BrdU阳性细胞表达情况。结果①神经功能缺损评分:模型组和经颅磁刺激组在脑出血后第1、7及28天时,差异无统计学意义;第14、21天评分分别为3.1±0.6、2.8±0.5和2.4±0.5、1.8±0.5,两组比较差异均有统计学意义(P<0.05)。②模型组和经颅磁刺激组大鼠血肿周围、室管膜下和海马区,均可见BrdU染色阳性细胞,对照组未见阳性细胞。脑出血后第7、14天,模型组和经颅磁刺激组BrdU染色阳性细胞分别为(32±10)、(47±...
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| 关 键 词： | 脑出血 经颅磁刺激 细胞增殖 大鼠 |
Effect of transcranial magnetic stimulation on the neuronal proliferation in rats with intracerebral hemorrhage |
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| LU Wang-sheng,TIAN Zeng-min,GUO Xin-ru,HUO Xiao-lin,LI Yue,LIU Shuang,WANG Ya-ming,YIN Feng.Effect of transcranial magnetic stimulation on the neuronal proliferation in rats with intracerebral hemorrhage[J].Chinese Journal of Cerebrovascular Diseases,2008,5(12). |
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| Authors: | LU Wang-sheng TIAN Zeng-min GUO Xin-ru HUO Xiao-lin LI Yue LIU Shuang WANG Ya-ming YIN Feng |
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| Institution: | LU Wang-sheng; TIAN Zeng-min; GUO Xin-ru; HUO Xiao-lin; LI Yue; LIU Shuang; WANG Ya-min; YIN Feng.Neurosurgical Center of PLA; Navy General Hospital of PLA; Beijing 100037; China; |
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| Abstract: | Objective To observe the effect of transcranial magnetic stimulation(TMS)on neurological functional recovery and neuronal proliferation in rats with intracerebral hemorrhage.Methods Forty-two healthy male SD rats were collected in the study,12 rats were randomly selected as the normal control group.A model of intracerebral hemorrhage(ICH)was established by injecting collagenase Ⅶ into the caudate nuclei of 30 rats,six rats were dead.Twenty-four rats were survived and randomly divided into a model group and ... |
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| Keywords: | Cerebral hemorrhage Transcranial magnetic stimulation Cell proliferation Rats |
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