| HPLC法测定消渴丸中葛根素的含量 |
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| 引用本文： | 魏玉德,唐海燕. HPLC法测定消渴丸中葛根素的含量[J]. 中国实用医药, 2009, 4(1): 24-25 |
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| 作者姓名： | 魏玉德 唐海燕 |
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| 作者单位： | 湖南省永州市药品检验所,425006 |
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| 摘 要： | 目的建立消渴丸中葛根素的含量测定方法。方法采用HPLC法，色谱柱为Hypersil BDS C18色谱柱，流动相为甲醇-水（25：75），流速为1．0ml／min，检测波长为250nm。结果进样量在0．0941～0．4705μg范围内线性关系良好（r=0．9997，n=5），平均回收率为101．0％，RSD为0．59％（n=5）。结论该方法操作简单，精密度高，重现性好，可作为控制消渴丸质量的方法。
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| 关 键 词： | 葛根素 含量测定 HPLC 消渴丸 |
Determination of pueranin in Xiaokewan by HPLC |
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| WEI Yu-de,TANG Hai-yan. Determination of pueranin in Xiaokewan by HPLC[J]. China Practical Medical, 2009, 4(1): 24-25 |
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| Authors: | WEI Yu-de TANG Hai-yan |
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| Affiliation: | .( Institute for Drug Control of Yongzhou City, Hunan Province 425006, China) |
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| Abstract: | Objective To determinate pueranin in Xiaokewan by HPLC. Methods Hypersil BDS C18 Column was used with methanol-water（25：75 ）as mobile phase, the flow rate was 1.0 ml/min, detection wavelength was set at 250 nm. Results Good linearity of pueranin was obtatined （ r = 0. 999 7, n = 5 ） with in the rang of 0. 094 1 -0. 470 5 μg. The average recovery was 101.0%, repeatability was good, and RSD was 0. 59% （ n = 5 ）. Conclusion The methodis simple, accurate and repeatabily, and can be used for the drug control. |
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| Keywords: | Pueranin Determinate HPLC Xiaokewan |
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