雷公藤多苷致小鼠卵巢早衰后的自然修复时间探讨

目的]考察雷公藤多苷造成小鼠卵巢早衰停药后能否自然修复及修复周期。方法]取健康小鼠25只,采用随机数字表方法取出5只作为正常对照组,其余作为模型对照组,实验小鼠灌胃给雷公藤多苷15 d后,通过外观观察;血清E2、P检测;阴道脱落细胞涂片;子宫、卵巢指数测定等,判断雷公藤多苷对小鼠卵巢的影响及停药后的自然修复情况。结果]雷公藤多苷片40 mg.kg-1/d灌胃,连续15 d能造成确切的雌性小鼠生殖功能低下状态,具体表现为,模型对照组与正常对照组比较,动情周期出现紊乱,血清雌二醇E2水平明显降低,与对照组相比,差异有显著性意义(P<0.05);孕酮P较正常对照组有降低趋势,但差异无显著性意义(P>0.05);子宫指数、卵巢指数均明显降低(P<0.05)。停药10 d后,模型组小鼠生殖功能呈自行恢复趋势,动情周期、性激素水平基本恢复正常。结论]雷公藤多苷口服连续灌胃15 d后可造成小鼠卵巢早衰,停药10 d后,卵巢功能即可自然修复。

Triptergium wifordii induced premature ovarian failure after the natural rehabilitation

Objective]To investigate the process of premature ovarian failure after Triptergium wilfordii(TW) treatment,whether the ovarian function in POF mice can natural recovery or not after the ending of treatment.Methods]After 15 days of TW treatment,we detected the serum level of E2 and P,and the indexes of uterus and ovarian.We also documented the smear of vaginal exfoliated cells.Results]All mice appeared poor status of physiological function.The cycle of estrus in the model group became disorder as compare...