冻干红细胞复水时体积响应显微观测及复水损伤机理探讨

冻干红细胞复水时造成的细胞损伤是目前红细胞冻干复水后回收率偏低的重要原因,主要表现复水时样品吸水,细胞环境溶液渗透压变化,细胞体积变化剧烈.了解复水时细胞体积响应对于了解冻干细胞复水损伤机理非常重要.本文中利用一种研究冻干细胞复水时体积响应的方法,即在环境扫描电镜下通过调整相对湿度来控制复水速度,显微跟踪观测细胞尺寸变化.结果 表明,在复水初始阶段红细胞厚度和直径先增大后减小,厚度方向变形能力和幅度比径向大.当体积偏移到一定程度时即发生溶血.因此为减小复水时溶血率,应控制复水条件、减小复水时细胞体积变化.

The microscopic observation of the volume response and the discussion of the rehydration injury about lyophilized erythrocytes during rehydration

The injury caused during rehydration is one of the most important reasons that lead to low recovery rate of freeze-dried erythrocytes after rehydration. During the rehydration process, the samples absorb water. The osmolality of cells environmental solution and the volume of cells change greatly. It is very important to analyse the rehydration injury mechanism by obtaining the volume response of cells during rehydration. In this paper, an alternative method to study the volume response of lyophilized cells was provided. Under the Environmental Scanning Electrical Microscopy (ESEM),the rehydration velocity was controlled by changing the relative humidity and the cells sizes were obtained during rehydration. The results implied that the thickness and the diameter of cells increased at the beginning stage of rehydration and decreased then. The changing magnitude and capacity in the thickness direction were greater than those of diameter. The hemolysis will occur when the volume excursion becomes serious. So it is very important to control the rehydration conditions and decrease the volume excursion of cells for avoiding hemolysis.