RP—HPLC法测定布洛芬-聚乙二醇6000固体分散体中布洛芬的含量

目的 建立反相高效液相色谱法测定布洛芬-聚乙二醇6000固体分散体中布洛芬的含量.方法 色谱柱为Shimadzu C18柱,流动相为0.02 mol·L^-1醋酸钠（冰醋酸调节pH至3.0±0.1）-乙腈（40：60）,流速1.0ml·min^-1,检测波长为264nm,进样量为20μl.结果 布洛芬质量浓度在0.051～1.02 mg·ml^-1范围内与相应峰面积呈良好线性关系（r=0.999 9,n=6）,平均加样回收率为98.40%,RSD为0.92%（n=9）.结论 所用方法准确、简便、快速,适用于布洛芬-聚乙二醇6000固体分散体中布洛芬的含量测定.

Use of RP-HPLC to detect the contents of ibuprofen in ibuprofen-PEG6000 solid dispersion

Objective To apply RP-HPLC to determine the contents of ibuprofen in ibuprofen-PEG6000 solid dispersion. Methods The separation was performed on a Shimadzu C18 column with UV detection at 264 nm, and the mobile phase consisted of 0.02 moLL-1 sodium acetate (adjusted to pH 3.0 ± 0.1 with acetic acid) -acetonitrile (40:60). The flow rate was 1.0 ml.min-1. The sample was injected at 20 μl.Results The calibration curve was linear (r = 0.999 9) within the range of 0.051 mg.ml-1 to 1.02 mg.ml-1 for ibuprofen. The average rate of retrieval was 98.40%, RSD was 0.92% (n = 9). Conclusions This method is accurate, simple, quick, and suitable for the quality control of Ibuprofen-PEG6000 solid dispersion.