脑梗死患者中性粒细胞与血管内皮细胞黏附性的研究

目的　分析脑梗死后外周血中性粒细胞 (PMN)与血管内皮细胞 (EC)黏附性的变化规律 ,探讨抗细胞间黏附分子 1单克隆抗体 (ICAM 1mAb)对其影响。方法　用细胞计数法检测 30例脑梗死患者梗死后 1周以内、2 1天时PMN与经 (或不经 )ICAM 1mAb处理的人类脐静脉内皮细胞细胞株ECV 30 4的黏附率。结果(1)脑梗死患者PMN与ECV 30 4的黏附率在 1周以内明显增高 ,到第 2 1天时显著下降 ;(2 )ICAM 1mAb可以明显降低 1周以内脑梗死患者PMN与ECV 30 4的黏附 (P <0 .0 1) ,而 2 1天时脑梗死患者和健康对照组PMN与ECV 30 4黏附则影响较小 (P <0 .0 5 )。结论　 (1)急性期脑梗死患者PMN与EC黏附增强 ,到恢复期时基本正常 ;(2 )ICAM 1参与介导脑梗死急性期PMN与EC的黏附 ,ICAM 1mAb可部分阻断PMN与EC黏附

The study of the adhesive properties of PMN and endothelial cells in patients with cerebral infarction

Objective To investigate the change of adhesive properties of polymorphonuclear neutrophils(PMN) and endothelial cells (EC) in patients with cerebral infarction (CI), and define the effects of antibodies to intercellular adhesion molecular 1 (ICAM 1, anti CD54 antibodies) upon the adhesion.Methods We detected the adhesive rate between human umbilical vein endothelial cells (ECV 304) and PMN of patients with CI within 1 week and at 21 days.Results (1) The adhesive rate of ECV 304 to PMN of 30 patients with CI within 1 week increased significantly ( P < 0 01), and decreased at 21 days. (2) Anti CD54 antibodies can reduce the adhesion between ECV 304 and PMN from patients of CI within 1 week ( P < 0 01), and have slightly effects to the patients at 21 days or normal controls ( P < 0 05).Conclusions (1) The adhesion between EC and PMN of patients in acute cerebral infarction increased, and became normal during recovery period. (2) The adhesion of PMN to EC can be mediated by ICAM 1, anti CD54 antibodies can partially block the adhesion between them.