| miR-126下调MMP-2抑制人脑胶质瘤细胞侵袭 |
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| 引用本文： | 陈寿仁,王占祥,沈上杭,谭国伟,刘希尧,朱宏伟.miR-126下调MMP-2抑制人脑胶质瘤细胞侵袭[J].肿瘤防治研究,2012,39(3):264-266. |
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| 作者姓名： | 陈寿仁 王占祥 沈上杭 谭国伟 刘希尧 朱宏伟 |
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| 作者单位： | 1.350004 福州，福建医科大学研究生教育学院；2.厦门大学附属第一医院神经外科 |
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| 基金项目： | 福建省自然科学基金面上项目资助课题(2009D002);厦门市科技局基金资助项目(3502220077055) |
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| 摘 要： | 目的初步探讨miR-126抑制人胶质瘤细胞侵袭的可能机制。方法化学合成miR-126，脂质体转染人脑胶质瘤U87细胞，应用RT-PCR、Western blot检测MMP-2基因和蛋白的表达情况，并应用Transwell小室检测转染前后细胞侵袭力的变化。结果miR-126上调后U87细胞的MMP-2基因和蛋白表达降低，并且细胞侵袭力明显降低。结论化学合成的miR-126在抑制人胶质瘤细胞侵袭过程中发挥重要作用，可能成为胶质瘤基因治疗的新靶点。
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| 关 键 词： | miR-126 胶质瘤 基质金属蛋白酶-2 侵袭 |
| 收稿时间： | 2011-06-03 |
miR-126 Inhibits Invasion of Human Brain Glioma Cells by Decreasing MMP-2 |
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| Chen Shouren
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Wang Zhanxiang
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Shen Shanghang
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Tan Guowei
,
Liu Xiyao
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Zhu Hongwei.miR-126 Inhibits Invasion of Human Brain Glioma Cells by Decreasing MMP-2[J].Cancer Research on Prevention and Treatment,2012,39(3):264-266. |
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| Authors: | Chen Shouren Wang Zhanxiang Shen Shanghang Tan Guowei Liu Xiyao Zhu Hongwei |
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| Institution: | 1.Fujian Medical University，College of Postgraduate education，Fuzhou 350004，China；2.Department of Neuro Surgery，The First Affiliated Hospital of Xiamen University |
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| Abstract: | Objective To explore the mechanism underlying the inhibiting effects of miR-126 on the invasion of human brain glioma cells.Methods miR-126 was synthesized and transfected into the U87 malignant glioma cells by liposome.The relative expression of MMP-2 and MMP-9 gene was quantified by RT-PCR,the expression of MMP-2 and MMP-9 protein was detected by Western blot,and cell invasion was assessed by Transwell chamber assay.Results miR-126 inhibited the activity of MMP-2 and the invasion of human U87 glioma cells.Conclusion The chemically synthesized miR-126 plays an important role in cell invasion of human brain glioma cells,which may be a promising target for glioma gene therapy. |
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| Keywords: | miR-126 Glioma MMP-2 Invision |
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