超声介导靶向微泡联合紫杉醇对卵巢癌细胞线粒体膜电位的影响

目的探讨超声介导LHRHa靶向微泡联合紫杉醇对人卵巢癌细胞株A2780/DDP线粒体膜电位的影响。方法采用生物素-链霉亲和素法合成LHRHa靶向微泡。将对数生长期的A2780/DDP细胞随机分为对照组、紫杉醇组、紫杉醇+超声组、普通微泡+紫杉醇+超声组、LHRHa靶向微泡组和LHRHa靶向微泡+紫杉醇+超声组。采用MTT法检测细胞增殖抑制率,激光共聚焦检测细胞线粒体膜电位变化。结果LHRHa靶向微泡+紫杉醇+超声组对细胞增殖抑制作用最明显,24h、48h和72h抑制率分别为(35.39±2.12)%、(66.90±2.15)%、(69.53±2.85)%,明显高于其他处理组(P均<0.05)。与对照组相比,除LHRHa靶向微泡组外,各处理组细胞线粒体膜电位均出现不同程度的降低,LHRHa靶向微泡+紫杉醇+超声组线粒体膜电位明显低于其他组(P均<0.05)。结论超声介导LHRHa微泡联合紫杉醇能有效增加紫杉醇对人卵巢癌细胞株A2780/DDP的增殖抑制和线粒体膜电位的耗散。

Impact of ultrasound mediate LHRHa targeted lipid microbubbles and Paclitaxel on the mitochondrial membrane potentialof ovarian carcinoma

Objective To observe the impact of ultrasound mediate LHRHa targeted lipid microbubbles and Paclitaxel on the mitochondrial membrane potential of ovarian carcinoma A2780/DDP cell.Methods LHRHa targeted microbubbles were prepared using biotin-avidin bridging method.A2780/DDP cells were divided into Paclitaxel,Paclitaxel plus ultrasound irradiation,lipid microbubbles combined Paclitaxel plus ultrasound irradiation,LHRHa targeted lipid microbubbles,LHRHa targeted lipid microbubbles combined Paclitaxel plus ultrasound irradiation and blank control groups.The ratio of cell growth inhibition of each treatment group was detected with MTT.Mitochondrial membrane potential was observed with confocal laser scanning microscopy.Results The cell proliferation in LHRHa targeted lipid microbubbles combined Paclitaxel plus ultrasound irradiation group was significantly inhibited,the inhibitory rate was(35.39±2.12)%,(66.90±2.15)%,and(69.53±2.85)% at 24 h,48 h,and 72 h,respectively,significantly higher than other groups at the same time point(all P<0.05).Compared with the control group,the mitochondrial membrane potential decreased in all of the treatment groups except LHRHa targeted lipid microbubbles group(all P<0.05).The mitochondrial membrane potential in the LHRHa targeted lipid microbubbles combined Paclitaxel plus ultrasound irradiation was lower than other treatment groups(all P<0.05).Conclusion Ultrasound mediate LHRHa microbubbles and Paclitaxel can inhibit the proliferation and depolarize the mitochondrial membrane potential of A2780/DDP cells.