| HPLC法测定己酮可可碱葡萄糖注射液中5-羟甲基糠醛限量 |
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| 引用本文： | 王继荣.HPLC法测定己酮可可碱葡萄糖注射液中5-羟甲基糠醛限量[J].实用医技杂志,2008,15(3):303-304. |
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| 作者姓名： | 王继荣 |
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| 作者单位： | 南京医科大学附属南京儿童医院,江苏,南京,210008 |
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| 摘 要： | 目的:采用HPLC法测定己酮可可碱葡萄糖注射液中5-羟甲基糠醛限量。方法:ODSC18柱(250×4.6mm,5μm),以甲醇:水:三乙胺(50:50:0.1)为流动相,检测波长为284nm。结果:回归方程A=4100.91+53467.52C(r=0.999,n=5),在浓度1μg/ml～20μg/ml的范围内线性良好,加样回收率为99.89%,RSD=0.19%。结论:本方法准确而灵敏,能有效地控制5-羟甲基糠醛的限量。
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| 关 键 词： | 己酮可可碱葡萄糖注射液 5-羟甲基糠醛 HPLC |
Determination of 5-hidroxymethylfurfural in pentoxifylline and glucose injection by HPLC |
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| WANG Ji-rong.Determination of 5-hidroxymethylfurfural in pentoxifylline and glucose injection by HPLC[J].Journal of Practical Medical Techniques,2008,15(3):303-304. |
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| Authors: | WANG Ji-rong |
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| Institution: | WANG Ji-rong ( The Nanjing Children＇s Hospital Affiliated to Nanjing Medical University, Jiangsu Nanjing 210008,China) |
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| Abstract: | Objective To determine 5-HMF in pentoxifylline and glucose injection. Methods C18 （250 mm×4.6 mm） column with methanol：water：triethylamine （50：50：0.1） as mobile phase was used. The detection wave-length was 284nm. Results The calibration curves of 5-hydroxymethylfurfural was linear in range of 1 μg/mL～20μg/mL. The average recovery was 99.89 %,（RSD=0. 19 %）.Conclusion The method is specific and reliable for the determination of 5-HMF in pentoxifylline and glucose injection. |
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| Keywords: | HPLC |
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