β-纤维蛋白原-455G/A基因多态性与缺血性脑血管疾病的相关性研究

目的研究β-纤维蛋白原-455G/A(-βFg-455G/A)基因多态性对血浆纤维蛋白原(fibrinogen,Fg)基因表达的影响及其与缺血性脑血管疾病发病的关系。方法应用聚合酶链反应(PCR)及限制性片段长度多态性分析(RFLP)等方法,检测DNA样品的-βFg-455G/A多态性,采用血浆自动检测系统测定Fg水平。结果病例组A等位基因频率明显高于对照组(P<0.05)。病例组血浆Fg水平明显高于对照组(P<0.01),病例组和对照组中,携带A基因者血浆Fg水平明显增高(P<0.05)。结论-βFg-455G/A等位基因与血浆纤维蛋白原水平相关,A基因携带者缺血性脑血管疾病发病率增高。提示-βFg-455G/A可作为缺血性脑血管疾病的遗传易感标志之一用于易感人群的检测。

Study on the relationship between β-Fibrinogen-455G/A Polymorphism and Ischemic Cerebral Vascular Disease

Objective To study the relationship between β-fibrinogen-455G/A polymorphism,plasma fibrinogen level and ischemic cerebral vacsular disease.Methods Study included 82 patients with ischemic cerebral vascular disase(P group) and 62 healthy people(C group).For all subjects,the polymorphism of β-fibrinogen-455G/A was analyzed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RELP) technique.Plasma fibrinogen was measured by a computer assistant procedure.Results The frequency of-455A allele in P group was higher than in C group(P<0.05).The plasma fibrinogen level was significantly higher in P group than in C group(P<0.01).Higher plasma fibrinogen level was shown in all subjects with A-455 alleles(P<0.05).Conclusions The β-fibrinogen-455G/A allele is associated with elevated fibrinogen level and those with A allele are prone to suffer ischemic cerebral vascular disease.