| 体外三维培养的结肠癌细胞整合素αⅤβ3表达变化及其与化疗敏感性的关系 |
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| 引用本文： | 郑晨宏,梁后杰,周琪,杨凤武,彭秋平,边志衡. 体外三维培养的结肠癌细胞整合素αⅤβ3表达变化及其与化疗敏感性的关系[J]. 肿瘤, 2008, 28(10). DOI: 10.3781/j.issn.1000-7431.2008.10.003 |
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| 作者姓名： | 郑晨宏 梁后杰 周琪 杨凤武 彭秋平 边志衡 |
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| 摘 要： | 目的: 用三维培养方法在体外模拟体内实体瘤生长情况,探讨结肠癌细胞群集耐药与细胞黏附分子整合素αⅤ(integrin αⅤ)和整合素β3(integrin β3)过度表达的关系.方法: 运用三维培养法获得人结肠癌细胞HT29多细胞球体(multicellular spheroids,MCS)模型,采用RT PCR法及免疫荧光法比较单层细胞(monolayer cells,MC)及MCS细胞中基质间黏附分子integrin αⅤ和integrin β3 mRNA及蛋白表达水平变化,用FCM法检测5 氟尿嘧啶(5 fluorouracil,5 FU)作用48 h后细胞的生存率和凋亡率.结果:HT29 MCS与HT29 MC经比较,integrin αⅤ和integrin β3 mRNA及蛋白表达水平均明显增高( P < 0.01),对5 FU的药物敏感性显著下降( P <0.05), 5 FU诱导的细胞凋亡率显著下降(0.346±0.035 vs 0.235±0.024),差异均有统计学意义( P <0.05).结论:细胞黏附分子integrin αⅤ和integrin β3表达水平增高可能增加结肠癌细胞群集的耐药性.
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| 关 键 词： | 结肠肿瘤 细胞培养技术 整合素αⅤ 整合素β3 抗药性 肿瘤 |
The altered expression of integrin αⅤβ3 in colon carcinoma cells cultured in three dimensional model and its relationship with sensitivity of tumor cells to chemotherapy |
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| ZHENG Chen-hong,LIANG Hou-jie,ZHOU Qi,YANG Feng-wu,PENG Qiu-ping,BIAN Zhi-heng. The altered expression of integrin αⅤβ3 in colon carcinoma cells cultured in three dimensional model and its relationship with sensitivity of tumor cells to chemotherapy[J]. Tumor, 2008, 28(10). DOI: 10.3781/j.issn.1000-7431.2008.10.003 |
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| Authors: | ZHENG Chen-hong LIANG Hou-jie ZHOU Qi YANG Feng-wu PENG Qiu-ping BIAN Zhi-heng |
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