| 不同来源血清和细胞因子对骨髓基质细胞成纤维细胞集落形成的影响 |
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| 引用本文： | 许力,毛平. 不同来源血清和细胞因子对骨髓基质细胞成纤维细胞集落形成的影响[J]. 内科, 2009, 4(2): 202-204 |
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| 作者姓名： | 许力 毛平 |
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| 作者单位： | 1. 广西壮族自治区人民医院血液科,南宁市,530021
2. 广州医学院附属广州市第一,人民医院血液科,510180 |
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| 摘 要： | 目的观察sD大鼠骨髓基质细胞（BMSC）体外培养的生物学特性并加以鉴定，并探讨不同来源血清和细胞因子对其成纤维系祖细胞（CFU-F）产率的影响。方法取SD大鼠骨髓，进行BMSC原代培养。传代后分别观察其生长特性，绘制贴壁率，测定生长曲线，并应用流式细胞仪鉴定细胞表型；采用体外长期液体培养法观察CFU—F的产率。结果原代培养的BMSCs生长良好，倍增时间约为40小时，传代后12小时贴壁率达85％以上；胎牛血清较脐血清和马血清能促进CFU-F的增值和分化。与未加细胞因子对照组相比，单用细胞因子SCF，SDF-1均可促进CFU—F的增殖，但二者之间差异无显著性（P〉0．05），同时加入SCF，SDF-1可明显的促进CFU-F的增殖，并优于单用SCF和SDF-1（P〈0．05）。结论体外培养的BMSCs生长稳定，传代后细胞适应性强；胎牛血清中可能较脐血清和马血清存在较多的对CFU—F有促进作用的生长因子；SCF可协同SDF-1促进CFU-F的增殖。
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| 关 键 词： | 骨髓基质细胞 血清 细胞因子 成纤维系集落形成单位 |
Effect of different serum and cytokines on the formation of bone marrow colony-forming unit-fibroblast |
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| XU Li,MAO Ping. Effect of different serum and cytokines on the formation of bone marrow colony-forming unit-fibroblast[J]. Internal Medicine of China, 2009, 4(2): 202-204 |
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| Authors: | XU Li MAO Ping |
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| Affiliation: | 1Department of Hematology;People's Hospital of Guangxi Autonomous Region;Nanning 530021;China;2Department of Hematology;the First People's Hospital of Guangzhou Medical College;Guangzhou 510180;China |
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| Abstract: | Objective To study and identify the biological characteristics of bone marrow stromal cells(BMSCs) of SD rats in vitro,and explore the effects of different serum and some cytokines on the yield of colony-forming unit-fibroblast(CFU-F) colony.Method BMSCs were isolated from bone marrow of SD miceand cultured in vitro,flow cytometry was used to identify the cells after cell passage and drawn adhesive rate and tested cell growth curve,long-term broth method was adopted to observe yield of CFU-F.Result BMSCs of... |
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| Keywords: | Bone marrow stromal cells Serum Cytokine Colony forming unit-fibroblast |
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