| 小鼠牙胚、软骨组织中牙本质涎磷蛋白基因的克隆 |
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| 引用本文： | 张蓉,肖明振,赵守亮,顾淑萍,岳玲,郑欣娟,汪平.小鼠牙胚、软骨组织中牙本质涎磷蛋白基因的克隆[J].牙体牙髓牙周病学杂志,2001,11(4):213-215. |
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| 作者姓名： | 张蓉 肖明振 赵守亮 顾淑萍 岳玲 郑欣娟 汪平 |
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| 作者单位： | 第四军医大学口腔医学院 |
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| 基金项目： | 国家自然科学基金资助项目(39800155)
国家教育部回国人员启动基金资助项目(2000HG003) |
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| 摘 要： | 目的：从小鼠牙胚、软骨组织中克隆牙本质涎磷蛋白基因（dentin sialophosphoprotein,DSPP)。方法：采用RT-PCR方法，从小鼠牙胚及软骨组织中克隆DSPP基因片段，将所得片段装入载体pGEM-TEasyVector进行序列测定。结果：从两种组织中均获得719bp的特异性片段，序列分析表明，与已发表的DSPP序列99.7%同源。结论：成功地从小鼠牙胚。软骨中克隆到DSPP基因部分序列。结果提示：除牙齿组织外，DSPP还可在软骨中表达，它可能并非是检测成牙本质细胞的特异性指标。
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| 关 键 词： | 软骨 牙本质涎磷蛋白 基因克隆 |
| 文章编号： | 1005-2593(2001)04-0213-03 |
| 修稿时间： | 2001年2月13日 |
Cloning of dentin sialophosphoprotein gene in mouse tooth germs and cartilage |
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| ZHANG Rong,XIAO Ming-zhen,ZHAO Shou-liang,et al.Cloning of dentin sialophosphoprotein gene in mouse tooth germs and cartilage[J].Chinese Journal of Conservative Dentistry,2001,11(4):213-215. |
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| Authors: | ZHANG Rong XIAO Ming-zhen ZHAO Shou-liang |
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| Abstract: | AIM:To clone DSPP gene from mouse tooth germs and cartilage.METHODS:DSPP gene was cloned from mouse tooth germs and cartilage by RT-PCR, both target fragments were inserted into commercial T Easy-Vector and sequenced. RESULTS: A 719bp fragment was obtained from the tooth germs and cartilage, respectively. The sequence analysis showed that they were 99.7% homologous to the published one.CONCLUSION: We successfully cloned partial sequence of DSPP from mouse tooth germs and cartilage. It suggested that DSPP could also be expressed in cartilage tissue except tooth tissue. It might not be regarded as the special marker of odontoblasts. |
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| Keywords: | Mouse cartilage Dentin sialophosphoprotein (DSPP) Gene cloning |
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