参葛方防治大鼠非酒精性脂肪肝的实验研究

目的:研究参葛方对CCl4复合高脂低蛋白饮食诱导的非酒精性脂肪肝模型大鼠的防治作用.方法:运用四氯化碳( carbon tetrachloride,CCl4)皮下注射4周(2次／周)复合高脂低蛋白饮食2周诱导非酒精性脂肪肝(non-alcoholic fatty liver,NAFL)动物模型,造模大鼠在造模第1天开始随机分为正常组、模型组、参葛方组及阳性药物对照组,自造模之日起参葛方高、中、低剂量组给药分别为0,10,5 g·kg-1,易善复组剂量为0.076 g·kg-1并ig给药或蒸馏水.处理后,观察药物对大鼠血清肝功能、肝脏组织病理变化、肝组织甘油三酯( triglyceride,TG)、游离脂肪酸(free fatty acid,FFA)含量,及丙二醛( malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)活性变化的影响.结果:与模型组相比,参葛方治疗组大鼠血清丙氨酸转氨酶( ALT,68.00±17.84,52.50±18.03)U·L-1,天冬氨酸转氨酶( AST,382.66±109.78,291.30±74.33)U·L-1显著降低(P＜0.01),肝组织脂肪变性明显改善,肝组织SOD活性有升高趋势,肝组织MDA( 370.25±61.28,249.82±15.98)nmo1·g-1含量显著下降(P＜0.01).结论:参葛方可显著降低CCl4结合高脂低蛋白饮食诱导的大鼠脂肪肝模型中TG 和FFA含量,减轻炎症损伤和肝脂肪变性,抗脂质过氧化作用,对NAFL动物模型有理想疗效.

Experimental Study on Prevention and Treatment by Shenge Formula in NAFL Model of Rats

Objective: To study the prevention and treatment action of Shenge formula on CCl₄ combined with high-fat and low-protein diet induced non-alcoholic fatty liver (NAFL) model. Method: The rat NAFL model was established by subcutaneous injection of carbon tetrachloride (CCl₄) for 4 weeks twice per week combined with high-fat and low-protein diet for 2 weeks. All rats were randomly grouped into normal group, model group, Shenge formula group and positive drug group. Distilled water and corresponding drugs were administered by intragastric administration method every day. After rats were sacrificed, the serum aminotransferase value, liver pathological changes and triglyceride(TG),free fatty acid(FFA) level, malondialdehyde(MDA)and superoxide dismutase (SOD) were examined. Result: Compared with model group, serum ALT and AST value were significantly decreased in Shenge formula group, and the MDA level in liver was significantly decreased in Shenge formula group(P<0.01). In addition, hepatic steatosis was significantly improved in Shenge formula. Conclusion: Shenge formula has protective effects on steatohepatitis, and it can significantly decrease the levels of TG and FFA, and improve inflammatory injury and hepatic steatosis, and anti-lipid peroxidation injury in the NAFL model.