应用单细胞凝胶电泳技术评价牛大力的遗传毒性

目的:应用单细胞凝胶电泳技术评价牛大力的遗传毒性.方法:40只小鼠随机分成5组:阳性对照组(环磷酰胺组)、阴性对照组(生理盐水组)、牛大力高、中、低( 20,10,5 g·kg-1)剂量组.牛大力各剂量组用牛大力煎煮液ig,阴性对照组、阳性对照组ig等量生理盐水,每天1次,连续10d,阳性对照组在最后2dip环磷酰胺(0.08 g·kg-1),每天1次,在最后1次给药6h后,处死各组小鼠,取肺、肾、肝、睾丸细胞,利用单细胞凝胶电泳技术观察牛大力对小鼠肺、肾、肝、睾丸细胞DNA的影响.结果:牛大力各剂量组对小鼠肺、肾、肝、睾丸细胞的尾部DNA百分率(Tail DNA％)和尾矩(Tail Moment)的影响明显低于阳性对照组,差异有统计学意义(P＜0.01),牛大力高剂量组肾细胞Tail DNA％与阴性对照组比明显降低,统计学上有显著差异(P＜0.05),其他各剂量组小鼠细胞tail DNA％和tail moment与阴性对照组比,差异无统计学意义.结论:牛大力对小鼠肺、肾、肝、睾丸细胞DNA未观察到明显损伤.

The Evaluation of Genotoxicity on Radix Millettiae Speciosae by Singe Cell Gel Electrophoresis

Objective: To evaluate genotoxicity of Radix Millettiae Speciosae by single cell gel electrophoresis. Method: Forty mice were divided into five groups randomly: negative control group, positive control group, and Radix Millettiae Speciosae group with high(20 g·kg^-1),middle (10 g·kg^-1)or low(5 g·kg^-1) doses. Radix Millettiae Speciosae groups were given with relative drugs, Negative control group and positive control group were given with normal saline by gavage once a day for 10 days, while the last two days, positive control group was injected with cyclophosphamide(0.08 g·kg^-1)once a day. In the eleventh day, the mice were sacreficed, DNA damage of liver, kidney, lung, testicular cells was detected by single cell gel electrophoresis. Result: Compared with positive control group, tail DNA% and tail moment of liver, kidney, lung, testicular cells in Radix Millettiae Speciosae group had a significant lowereduction (P<0.01).Compared with the negative control group, tail DNA%and tail moment of kidney cells in the Millettiae Speciosae group with high dose showed a significant reduction (P<0.05), tail moment and tail DNA% of ther cells in other dose group, no statistically significant difference was found. Conclusion: Radix Millettia Speciosa has no damage to mouse cells DNA.