温石棉对兔肺泡巨噬细胞合成一氧化氮及抗氧化酶活性的影响

为探讨一氧化氮及抗氧化酶在石棉致病中的作用，用改良Ｍｙｒｖｉｋ法收集兔肺泡巨噬细胞（ＡＭ）进行体外培养，采用硝酸还原酶法测定ＵＩＣＣ温石棉处理后兔ＡＭ培养液中亚硝酸根（ＮＯ＾－２）的含量（ＮＯ的静态氧化形式），同时利用黄嘌呤及黄嘌呤氧化酶反应系统测定ＡＭ的细胞裂解液中ＳＯＤ的活性，和酶－底物反应形成有色物质来测定ＡＭ的细胞裂解液中ＮＯＳ和ＧＳＨ－Ｐｘ的酶活性。

Effect of chrysotile on nitric oxide production and anti-oxidasic activity in rabbit alveolar macrophages]

This study aimed to explore the role of nitric oxide (NO) and nitric oxide synthase (NOS) in chrysotile asbestos-caused diseases. The production of NO2-/NO3- (the final product of NO) and the alterations of NOS, SOD and GSH-Px activity were investigated when rabbit alveolar macrophages (AM) were stimulated by UICC chrysotile. The results revealed that with the dosage elevation of UICC chrysotile, the rabbit alveolar macrophages showed: (1) increased mortality, decreased survival activity; (2) increased product of NO2-/NO3-, decreased SOD and GSH-Px activity; (3) increased NOS activity in the lower dosage groups, but decreased NOS activity in the higher dosage groups. There was significantly negative correlation between NO release and SOD (or GSH-Px) activity (r1 = -0.7125, P < 0.05; r2 = -0.8496, P < 0.05 respectively). Moreover, the significantly positive correlation between NO release and NOS activity was found in the lower dosage groups, but significantly negative correlation in the higher dosage groups. These findings suggest that chrysotile could induce the alveolar macrophages to increase NO release and to decrease SOD and GSH-Px activity, which may play a role in the process of asbestos-caused diseases.