C57/BL6小鼠母婴分离模型中医证型探讨

目的?探讨C57/BL6小鼠母婴分离（MS）模型中医证型。方法?通过母婴分离干预新生鼠，采用旷场实验评价母婴分离对小鼠自发活动的影响，强迫游泳和强迫悬尾实验评价母婴分离对小鼠不动时间的影响。四诊计量化指标检测与计量辨证方法探讨小鼠气血阴阳状态。通过促肾上腺皮质激素（ACTH）、皮质酮（CORT）测定，观察下丘脑-垂体-肾上腺皮质轴（HPA）功能状态。通过环磷酸腺苷/环磷酸鸟苷（cAMP/cGMP）、三磷酸腺苷酶（ATPase）及超氧化物歧化酶（SOD），探讨能量代谢水平。结果?旷场实验中各组动物活动总路径差异无统计学意义（P>0.05）。MS180组在强迫游泳实验、强迫悬尾实验中，与对照组及MS15组比较小鼠不动时间显著增加（P<0.01）。MS180组阳热水平较对照组和MS15组显著降低（P<0.01）。MS180组ACTH及CORT值升高，与对照组及MS15组比较有显著性差异（P<0.01）。MS180组cAMP/cGMP降低，与对照组和MS15组比较有显著性差异（P<0.01）。MS180组Na+-K+-ATPase活性降低，与对照组和MS15组比较有显著性差异（P<0.01）。MS180组SOD活性降低，与对照组和MS15组比较有显著性差异（P<0.01）。结论?母婴分离对小鼠行为有显著影响，小鼠出现抑郁样行为，小鼠HPA轴功能受到影响，机体能量代谢水平降低，小鼠表现出的证候特征属于中医阳虚证。 

Investigation of TCM Syndromes on Maternal Separation in C57/BL6 Mice

OBJECTIVE?To investigate TCM syndromes on maternal separation in C57/BL6 mice. METHODS?Neonatal mice were intervened through maternal separation. The effects of maternal separation on spontaneous activities of mice were evaluated by field test and the effects on immobility times of mice were evaluated by forced swimming test and tail suspension test. Four diagnostic quantitative indicators measurement and quantitative dialectical method were employed to explore the state of Qi， Blood， Yin and Yang in mice. Hypothalamus-pituitary-adrenal (HPA) cortical axis function was observed through adrenocorticotropic hormone (ACTH) and corticosterone (CORT) determination. The level of energy metabolism was investigated through the measurement of cyclic adenosine monophosphate/cyclic guanosine monophosphate (cAMP/cGMP)， ATPase and superoxide dismutase(SOD). RESULTS?No statistical significance (P>0.05) was observed in the total path of animal activities between each group in field test. Compared with the control group and MS15 group， in the forced swimming test and tail suspension test， the immobility time of mice of MS18 group was significantly increased (P<0.01). The levels of Yang heat， cAMP/cGMP， Na+-K+-ATPase and SOD significantly decreased (P<0.01)， while ACTH and CORT significantly increased (P<0.01) of MS18 group， compared to the control group and MS15 group. CONCLUSION?Maternal separation shows significant effects on behavior in mice. The mice exhibit depression-like behavior， upset HPA axis balance and reduce body energy metabolism， which characterized as Yang deficiency symptoms in TCM.