四角蛤蜊粗多糖对四氧嘧啶诱导ICR小鼠糖尿病模型的降血糖作用

目的?研究四角蛤蜊粗多糖中具有降血糖作用的活性部位。方法?ICR小鼠，尾静脉注射四氧嘧啶(80?mg/kg）生理盐水溶液，96?h后筛选空腹血糖值≥11.1?mmol/L的糖尿病小鼠。四角蛤蜊粗多糖1按200、400、800、1?200?mg/kg剂量进行分组。分别测定第7、14天的空腹血糖值，糖化血清蛋白、总胆固醇及甘油三酯含量等指标。另取糖尿病小鼠，按照筛选出四角蛤蜊粗多糖1的最佳剂量（800?mg/kg），将四角蛤蜊粗多糖1按醇沉范围分段，相同生药量换算剂量，分为四角蛤蜊粗多糖2、3组。测定四角蛤蜊粗多糖1组（800?mg/kg）、四角蛤蜊粗多糖2组（180、360?mg/kg）和四角蛤蜊粗多糖3组（2?040?mg/kg）给药第7天空腹体质量和血糖水平。结果?四角蛤蜊粗多糖1具有降血糖作用，可降低小鼠糖化血清蛋白、总胆固醇及甘油三酯含量，其中四角蛤蜊粗多糖2（180、360?mg/kg）具有降血糖作用，四角蛤蜊粗多糖3无降血糖作用。结论?四角蛤蜊粗多糖1具有降血糖作用，最佳剂量为800?mg/kg，其降糖活性部位集中于四角蛤蜊粗多糖2。 

The Anti-hyperglycemic Activity of Crude Polysaccharides from Mactra Veneriformis in Alloxan Induced Diabetes in Mice

OBJECTIVE?To study the anti-hyperglycemic effective sites of crude polysaccharides from Mactra veneriformis (MVPS). METHODS? Hyperglycemia models were established by intravenous injection of alloxan via tail vein of ICR mice under a dose of 80?mg/kg. After 96?h， mice with fasting blood glucose levels≥11.1?mmol/L were chosen and divided into groups. MVPS 1 treated at the doses of 200， 400， 800?mg/kg and 1?200?mg/kg once daily， respectively. At 7th and 14th days， respectively， the levels of blood glucose， GSP， TCH and TG were measured. Other diabetic mice were divided into MVPS 1 (800?mg/kg)， MVPS 2 (180， 360?mg/kg) and MVPS 3 (2?040?mg/kg) groups. After 7 d treatment， both body weights and fasting blood glucose levels were measured. RESULTS?MVPS 1 of 800?mg/kg displayed the optimal anti-hyperglycemic effect on diabetic mice and reduced GSP， TCH and TG levels of diabetic mice; MVPS 2 showed significantly anti-hyperglycemic activities， compared to the model group. MVPS 3 exhibited no anti-hyperglycemic activities. CONCLUSION?MVPS 1 displays the optimal hypoglycemic effect at the dose of 800?mg/kg， and its hypoglycemic active site focuses on MVPS 2.