人工虫草多糖对2型糖尿病小鼠胰岛素抵抗的影响

目的 观察人工虫草多糖对2型糖尿病小鼠胰岛素抵抗的影响及作用机制. 方法 采用高脂饲料(fat-fed,FF)联合腹腔注射小剂量链脲佐菌素(STZ,25 mg•kg^-1)建立胰岛素抵抗2型糖尿病模型(T2DM-IR),每日给予不同剂量人工虫草多糖(200,400 mg•kg^-1)治疗,连续28 d. 观察实验小鼠体质量、空腹血糖的变化. 末次给药前小鼠禁食12 h,测定口服糖耐量. 随后眼球取血,检测血清胰岛素、三酰甘油(TG)、总胆固醇(TC)、游离脂肪酸(FFA),计算胰岛素抵抗指数(HOMA-IR). 结果FF/STZ诱导的实验性小鼠,出现以腹型肥胖、血糖及胰岛素水平异常等T2DM-IR典型症状. 人工虫草多糖可剂量依赖性地改善糖尿病小鼠糖耐量及胰岛素抵抗指数,同时降低血清胰岛素、TG、TC、FFA水平. 结论 400 mg•kg^-1人工虫草多糖可明显降低FF/STZ诱导的T2DM-IR模型小鼠血糖与血脂,改善胰岛素抵抗,作用机制可能与增强胰岛素受体敏感性有关.

Effect of Polysaccharides from Cultured Mycelium of Cordyceps sinensis on Insulin Resistance T2DM Mice

Objective To investigate effects of polysaccharides from cultured mycelium of Cordyceps sinensis(Berk.) Sacc.(PCS) on insulin resistance in a fat-fed(FF) and streptozotocin(STZ)-induced type 2 diabetic mice model.Methods Normal male Balb/c mice were i.p.injected with low-dose of STZ for consecutive 3 d and fed with high fat diet for 3 weeks simultaneously.PCS(200,400 mg.kg-1) were administered orally to FF/STZ induced diabetic mice for 28 d.An oral glucose tolerance test(OGTT) followed the changes of fastin...