| 三七对酒精性肝病大鼠肝组织CYP2E1表达的影响 |
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| 引用本文： | 吴黎艳,陈芝芸,严茂祥,何蓓晖. 三七对酒精性肝病大鼠肝组织CYP2E1表达的影响[J]. 中华中医药杂志, 2011, 0(6): 1395-1398 |
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| 作者姓名： | 吴黎艳 陈芝芸 严茂祥 何蓓晖 |
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| 作者单位： | 浙江中医药大学;浙江省中医院; |
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| 基金项目： | 浙江省中医药科技计划资助项目(No.2007YA009)~~ |
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| 摘 要： | 目的:观察三七对酒精性肝病大鼠肝组织CYP2E1表达及MDA含量、SOD活性的影响,探讨其防治酒精性肝病的作用机制。方法:采用白酒-玉米油-吡唑混合液连续灌服14周建立酒精性肝病模型,同时以高、低剂量的三七粉和硫普罗宁进行干预14周。免疫组化Envision法检测肝组织CYP2E1表达,黄嘌呤氧化酶法检测肝组织匀浆SOD活性,硫代巴比妥酸法检测MDA含量。结果:造模4周组织CYP2E1表达、MDA含量及SOD活性与同期正常组比差异无显著性;随酒精造模时间的延长,大鼠肝组织CYP2E1表达增强,MDA含量增加,SOD活性降低,造模8周、14周时CYP2E1表达和MDA含量较同期正常组明显增加(P<0.05,P<0.01),SOD活性则较同期正常组明显降低(P<0.05,P<0.01),且CYP2E1表达水平与MDA呈明显正相关(r=0.458,P<0.01),与SOD呈明显负相关(r=-0.541,P<0.01);应用三七和硫普罗宁干预14周后,CYP2E1表达和MDA含量较模型14周组明显下降,SOD活性较模型14周组明显增强。结论:三七能够显著的抑制酒精性肝病大鼠CYP2E1的表达,减轻脂质过氧化反应,这可能是其防治酒精性肝病的作用机制之一。
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| 关 键 词： | 酒精性肝病 细胞色素P4502E1 脂质过氧化 三七 |
Effect of Sanchi on expression of hepatic CYP2E1 in liver tissues in rats with alcoholic liver disease |
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| WU Li-yan,CHEN Zhi-yun,YAN Mao-xiang,HE Bei-hui. Effect of Sanchi on expression of hepatic CYP2E1 in liver tissues in rats with alcoholic liver disease[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2011, 0(6): 1395-1398 |
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| Authors: | WU Li-yan CHEN Zhi-yun YAN Mao-xiang HE Bei-hui |
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| Affiliation: | WU Li-yan1,CHEN Zhi-yun2,YAN Mao-xiang2,HE Bei-hui2(1Zhejiang University of TCM,Hangzhou 310006,China,2Zhejiang TCM Hospital,China) |
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| Abstract: | Objective: To investigate the effect of sanchi on the expression of CYP2E1 ,the contents of MDA and the activity of SOD in liver of rats with alcoholic liver disease, and explore the mechanism of sanchi in preventing from alcoholic liver disease . Methods: the model of alcoholic liver disease in rats was induced with mixture of alcohol-corn oil-pyrazole in 14 weeks. Rats in low dose sanchi i, high dose sanchi and Tiopronin groups were treated with sanchi and Tiopronin during the 14 weeks. Expression of CYP2... |
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| Keywords: | Alcoholic liver disease CYP2E1 Lipid peroxidation Sanchi |
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