| 桃仁、木香、黄芪分别与红花配伍对大鼠早期桡骨骨折愈合过程中BMP-2表达的影响 |
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| 引用本文： | 王轩,潘琪,张慧萍,李引刚. 桃仁、木香、黄芪分别与红花配伍对大鼠早期桡骨骨折愈合过程中BMP-2表达的影响[J]. 中华中医药杂志, 2011, 0(6): 1423-1425 |
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| 作者姓名： | 王轩 潘琪 张慧萍 李引刚 |
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| 作者单位： | 北京中医药大学基础医学院;山西中医学院中西医结合临床医学系;兴平市妇幼保健院外科;山西省中西医结合医院心内科;陕西中医学院附属医院骨科; |
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| 基金项目： | 咸阳市科技计划项目(No.XK06015-5)~~ |
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| 摘 要： | 目的:研究桃仁、木香、黄芪分别与红花配伍对实验性SD大鼠早期桡骨骨折愈合过程中骨痂厚度及BMP-2表达的影响,探讨骨折三期辨证的治疗方法。方法:256只SD大鼠造模后随机分为模型组、桃仁红花组、木香红花组、黄芪红花组4组,每组各64只,每组再随机平均分为4个小组,分别作为骨折后第3、7、10、14d组,每小组16只。于造模后第3、7、10、14d,处死1小组大鼠。取材进行常规HE染色观察骨痂厚度、BMP-2-DAB显色计数观察BMP-2阳性细胞数。结果:造模后第3d,各给药组与模型组比较,标本BMP-2阳性细胞数及骨痂厚度差异无统计学意义;造模后第7、10、14d,各给药组与模型组比较,标本BMP-2阳性细胞数及骨痂厚度差异有统计学意义(P<0.05,P<0.01),桃仁红花组、黄芪红花组与木香红花组比较,标本BMP-2阳性细胞数及骨痂厚度差异有统计学意义(P<0.05)。结论:木香与红花配伍能显著提高SD大鼠早期桡骨骨折断端局部BMP-2的表达水平,增加骨痂厚度,就促进SD大鼠骨折愈合过程中BMP-2表达作用而言,理气活血法可能是四肢闭合骨折早期的较佳治法。
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| 关 键 词： | 桡骨骨折 骨形态发生蛋白2 免疫组织化学 |
Effects of different compatibility of TCM on BMP-2 expression at fracture site and healing of early radius fracture of rats |
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| Affiliation: | WANG Xuan,PAN Qi,ZHANG Hui-ping,LI Yin-gang( Preclinical Medicine School,Beijing University of Chinese Medicine,Beijing ,China, Clinical Science of Integrative Medicine,Shanxi University of TCM,Taiyuan , Department of Surgery,Xingping Women , Children’s Hospital , Health Institute,Xingping ,Department of Cardiology Shanxi Hospital of Integrated Traditional , Western Medicine,Taiyuan ,Department of Orthopedics,Affiliated Hospital of... |
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| Abstract: | Objective: To observe the effect of different compatibility of herbs on BMP-2 expression at fracture site and thickness of callus of early radius fracture rat models. Methods: 256 SD rats were divided into four groups randomly: model group, Semen Persicae and Flos Carthami group, Radix Aucklandiae and Flos Carthami group, Radix Astragali and Flos Carthami group. Left radius fracture rat models were established. Conventional haematoxylin and eosin staining and immunostaining for bone section were carried out... |
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| Keywords: | Radius fracture BMP-2 Immunohistochemistry |
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