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人参多糖含量测定方法研究
引用本文:王丹,吕永磊,徐丽媛,卜海博,李向日.人参多糖含量测定方法研究[J].中华中医药杂志,2011(4):774-776.
作者姓名:王丹  吕永磊  徐丽媛  卜海博  李向日
作者单位:北京中医药大学中药学院;
基金项目:国家自然基金面上项目(No.81073041)~~
摘    要:目的:建立人参多糖含量测定方法。方法:以人参精制多糖测得人参多糖对葡萄糖的换算因子,以苯酚-硫酸分光光度法测定人参多糖的含量。结果:供试液在2h内显色稳定,重现性好,平均回收率为99.8%,RSD为2.57%。人参多糖含量为28.56%,RSD为1.81%。结论:该方法简便,快速,测定结果客观,准确。

关 键 词:人参  多糖  含量测定  精制

Content Determination of Polysaccharide in the root of Panax ginseng
WANG Dan,LV Yong-lei,XU Li-yuan,BU Hai-bo,LI Xiang-ri.Content Determination of Polysaccharide in the root of Panax ginseng[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(4):774-776.
Authors:WANG Dan  LV Yong-lei  XU Li-yuan  BU Hai-bo  LI Xiang-ri
Institution:WANG Dan,LV Yong-lei,XU Li-yuan,BU Hai-bo,LI Xiang-ri(School of Chinese Materia Medica,Beijing University of Traditional Chinese Medicine,Beijing 100102,China)
Abstract:Objective:To establish a method for content determination of polysaccharides in the root of Panax ginseng.Method:The conversion coefficient of ginseng polysaccharide to glucose was obtained by refined polysaccharides,and then the content of crude polysaccharides in the root of Panax ginseng was determined by the method of phenol-sulferic acid.Result:the color of the treated solution showed good stabilization within 2h and the average value of the recovery for polysaccharide measurement was 99.8% with 2.57% ...
Keywords:Panax ginseng  Polysaccharide  Content determination  Refining  
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